The characterization of fibrous sheath and outer dense fiber proteins of the rat sperm tail

Simple item page
dc.contributor.advisorvan der Hoorn, Frans A.
dc.contributor.authorFitzgerald, Carolyn
dc.date.accessioned2005-08-10T16:01:25Z
dc.date.available2005-08-10T16:01:25Z
dc.date.issued2004
dc.descriptionBibliography: p. 116-124en
dc.descriptionSome pages are in colour.en
dc.description.abstractThe aim of this study was to further characterize three prominent proteins of the rat sperm tail, ODF14, FS14 and Spag5. FS14 and ODF14 were used to generate antibodies which were subsequently affinity-purified and used to screen a rat testicular cDNA library. Two cDNAs were isolated (designated 1017 and 1038) and it was determined that 1017 was homologous to mouse ornithine decarboxylase antizyme 3. However, it was found that 1038 resulted from a chimeric mRNA, consisting of sequences from two different chromosomes. Spag5 was originally thought to be testis-specific and was identified due to its interaction with Odf1 using the yeast two hybrid system. Knock-out studies revealed no significant phenotype in mice and spag5 was found to be 73% similar to the human mitotic spindle protein, Astrin. We found that in spermatogenesis Spag5 does not appear to localize to either the axoneme or the meiotic spindle, but instead associates with ODF. In mitotic cells it colocalizes with Astrin in the mitotic spindle and the cytoplasm of rat IEC-18 cells.en
dc.format.extent3093008 bytes
dc.format.extentvii, 124 leaves : ill. ; 30 cm.en
dc.format.mimetypeapplication/pdf
dc.identifier.citationFitzgerald, C. (2004). The characterization of fibrous sheath and outer dense fiber proteins of the rat sperm tail (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/20576en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/20576
dc.identifier.urihttp://hdl.handle.net/1880/41333
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyMedicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsAttribution Non-Commercial No Derivatives 2.5*
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/*
dc.titleThe characterization of fibrous sheath and outer dense fiber proteins of the rat sperm tail
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thesis.pdf
Size:
2.95 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Legacy Theses