Micro-environmental factors directing differentiation of murine embryonic stem cells down osteogenic and chondrogenic lineages

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atmire.migration.oldid747
dc.contributor.advisorDuncan, Neil
dc.contributor.advisorKrawetz, Roman
dc.contributor.authorHazenbiller, Olesja
dc.date.accessioned2013-02-15T20:27:10Z
dc.date.available2013-06-15T07:01:49Z
dc.date.issued2013-02-15
dc.date.submitted2013en
dc.description.abstractThe field of bone tissue engineering (BTE) aims to develop graft substitutes for diseased or difficult to heal fractures. We used a BTE construct made of collagen type I and murine embryonic stem cells (mESCs) which has been shown to trigger mESCs differentiation into osteoblasts, and successfully contribute to fracture repair in vivo within a mouse model system. Bone healing is a complex process involving the interplay of biochemical and biomechanical cues. Therefore, this project aimed to systematically emulate the roles of chemical and mechanical cues present during fracture repair on the differentiation of mESCs in vitro in order to optimize treatment strategies for BTE. Further characterization of this cell/gel construct revealed that mESCs differentiate into a heterogeneous cell population of chondrocytes and osteoblasts, replicating the process of endochondral ossification that normally occurs during fracture repair. To study the effect of biomechanical cues, a loading system was specifically designed and characterized to apply confined compressive load to a soft, viscoelastic cell/gel construct. Mechanical stimuli enhanced chondrogenic differentiation but had no effect on osteogenic differentiation. Moreover, the role of integrins in directing mESCs differentiation and transducing mechanical signals was evaluated. Finally, the synergistic effect between extracellular matrix mediated differentiation, mechanical stimulation and BMP-2 delivery to the system using nano-particles was studied.en_US
dc.identifier.citationHazenbiller, O. (2013). Micro-environmental factors directing differentiation of murine embryonic stem cells down osteogenic and chondrogenic lineages (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26906en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26906
dc.identifier.urihttp://hdl.handle.net/11023/554
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultySchulich School of Engineering
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEngineering--Biomedical
dc.subject.classificationtissue engineeringen_US
dc.subject.classificationEmbryonic stem cellsen_US
dc.subject.classificationDifferentiationen_US
dc.titleMicro-environmental factors directing differentiation of murine embryonic stem cells down osteogenic and chondrogenic lineages
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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