A Biophysical Characterization of Histone detived Antimicrobial Peptides

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atmire.migration.oldid1410
dc.contributor.advisorPrenner, Elmar
dc.contributor.authorBerezowski, Neil Steven
dc.date.accessioned2013-09-23T20:12:01Z
dc.date.available2013-11-12T08:00:17Z
dc.date.issued2013-09-23
dc.date.submitted2013en
dc.description.abstractHistones have been recently shown to be integral to the response of the body to invading pathogens. As such, prior work on this project identified segments of several histones or fragments thereof as potential AMPs. This project comprises a continued biophysical characterization of these selected segments of histone. The project investigates the first generation of AMPs with monolayer models and Brewster angle microscopy to screen for the lipids which are most important in AMP-lipid interactions. Subsequently new variant peptides were designed from one parent peptide (H3) and studied through the use of several thermodynamic and spectroscopic techniques. The variant peptides were shown to be more potent than the parent peptide in terms of microbicidal activity, but were not as selective for bacterial membranes. Therefore, further studies are needed to determine the therapeutic value of the variants.en_US
dc.identifier.citationBerezowski, N. S. (2013). A Biophysical Characterization of Histone detived Antimicrobial Peptides (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24634en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/24634
dc.identifier.urihttp://hdl.handle.net/11023/995
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subjectGeneral
dc.subject.classificationAntimicrobial peptidesen_US
dc.subject.classificationMembranesen_US
dc.subject.classificationLipidsen_US
dc.subject.classificationIsothermal Titration Calorimetryen_US
dc.subject.classificationDifferential Scanning Calorimetryen_US
dc.subject.classificationCircular Dichroismen_US
dc.subject.classificationCalcein Leakageen_US
dc.subject.classificationFluorescenceen_US
dc.subject.classificationDynamic Light Scatteringen_US
dc.titleA Biophysical Characterization of Histone detived Antimicrobial Peptides
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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