Development of a Redox Functional Nucleic Acid Integrated Electrochemical Biosensors for Wearable Applications
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2024-01-19
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Abstract
The corrugated nature of biosensor surfaces holds paramount significance in increasing their efficiency. This investigation scrutinizes the electrochemical performance of wrinkled and flat sensor surfaces. The textured surface is embedded with wrinkles about 1 μm in thickness and a wavelength of 32 μm. Testing the biosensors with a 1μM cortisol sample, the wrinkled electrode produced a fold change of 1.84±0.65 compared to the planar electrode, which produced a fold change of -0.11±0.07. Such observations underscore the heightened signal augmentation of the textured design. Additionally, specificity tests for the cortisol concatenated aptamer showed no false positive signal when tested with other interferent biomarkers. Initial trials utilizing cortisol mixed artificial sweat and DI samples demonstrated its performance in cortisol detection with an LOD of 59 nM in artificial sweat. The assay's reusability was tested through multiple incubations with a standard cortisol solution, indicating consistent performance over three cycles. Stability trials in artificial sweat revealed a stable initial response, maintaining stability for up to six hours before a notable decline after 72 hours. However, further experiments may be required to prepare the device for more practical applications. Attempts to regenerate the biosensor using UV light were unsuccessful, and further on-body and human sweat tests showed reduced effectiveness compared to laboratory conditions. While the initial development, optimization and testing of the biosensor achieved some successes, it also highlighted several areas requiring refinement, including but not limited to electrode design, standardization of testing procedures, and optimization of the heating process to enhance the functionality and reliability of the biosensor for its intended applications.
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Janghorban, M. (2024). Development of a redox functional nucleic acid integrated electrochemical biosensors for wearable applications (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.