Genetic and biochemical aspects of trehalase and sucrase of Drosophila Melanogaster

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dc.contributor.advisorWilliamson, John H.
dc.contributor.authorOliver, Melvin J. (Melvin John), 1951-
dc.date.accessioned2005-07-19T20:21:01Z
dc.date.available2005-07-19T20:21:01Z
dc.date.issued1977
dc.descriptionBibliography: p. 72-78.en
dc.description.abstractThere are three major forms of the enzyme trehalase (E.C~ 3.2.1.28) found in Drosophila melanogaster, a soluble abdominal form, a soluble muscle form and an insoluble) particulate, muscle form c The location of an area of the genome coding for the production of all three forms of the enzyme is proposed on the basis of enzyme determinations in a set of duplication and deletion bearing aneuploids exhibiting dosage sensitivity. This region lies between 55B and 55E on the right arm of chromosome two of the Drosophila genome. The localization of the structural gene of the enzyme sucrase (E.C. 3.2.1.26) is proposed on the basis of similar determinations. The sucrase structural gene lies between 31CD and 31EF on the left arm of chromosome two of the Drosophila genome. Polyacrylamide disc-gel electrophoresis reveals only one band of trehalase activity in crude homogenates of whole fly, thorax and abdomen preparations. Similarly only one peak of trehalase activity is seen when these extracts are run on isoelectrofocusing columns. Drosophila trehalase has an isoelectric point of 4.73 ± 0.02. Isoelectrofocusing of abdominal extracts reveals the existence of two sucrase components, one exhibiting an isoelectric point of 4.63 ± 0.02 and the other an iso electric point of 4.83 ± 0.02. This suggests that there are two forms of abdominal sucrase in Drosophi la. On the basis of these data it is concluded that the three forms of trehalase are manifestations of a single protein whose primary sequence is coded for by a single trehalase gene, designated Treh+. Similarly the two forms of abdominal sucrase derive from a common protein coded for by a single sucrase gene, designated Sucr+.
dc.format.extentx, 78 leaves : ill. ; 30 cm.en
dc.identifier82481022en
dc.identifier.citationOliver, M. J. (1977). Genetic and biochemical aspects of trehalase and sucrase of Drosophila Melanogaster (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/15946en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/15946
dc.identifier.lccQH 470 D7 O45 1977 Microficheen
dc.identifier.other82481022en
dc.identifier.urihttp://hdl.handle.net/1880/13816
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject.lccQH 470 D7 O45 1977 Microficheen
dc.subject.lcshFruit flies
dc.subject.lcshBiochemical genetics
dc.subject.lcshTrehalase
dc.subject.lcshSucrase
dc.titleGenetic and biochemical aspects of trehalase and sucrase of Drosophila Melanogaster
dc.typemaster thesis
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopyTRUE
ucalgary.thesis.accessionTheses Collection 58.002:Box 302 82481022
ucalgary.thesis.notesPLen
ucalgary.thesis.uarcreleasenoen
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