Characterization of a PDGF-AA Initiated Model of Glioblastoma with Predictable Onset

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atmire.migration.oldid3280
dc.contributor.advisorCairncross, J. Gregory
dc.contributor.authorDePetro, Jessica
dc.date.accessioned2015-05-27T15:47:14Z
dc.date.available2015-11-20T08:00:26Z
dc.date.issued2015-05-27
dc.date.submitted2015en
dc.description.abstractGlioblastoma multiforme is a fatal brain cancer which has eluded therapeutic advances. Although the genetic and epigenetic landscape of GBM is now well annotated, its pathogenesis remains elusive and difficult to study in the laboratory. By imitating two well documented alterations of GBM - p53 loss of function and activation of platelet derived growth factor receptor-alpha - we have created a new model of GBM and a tool to explore the kinetics of GBM initiation. In this model, cells from the subventricular zone of p53 null mice transform in vitro after approximately 100 days in PDGF-AA. Transformation is marked by abrupt, rapid proliferation, exogenous PDGF-AA independence, and the capacity to form invasive GBM-like tumors in p53 wild type, immunocompetent mice. This model offers the opportunity to study the nature and sequence of transformation-related events and potentially test novel therapeutics that target GBM in its earliest stages.en_US
dc.identifier.citationDePetro, J. (2015). Characterization of a PDGF-AA Initiated Model of Glioblastoma with Predictable Onset (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25269en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25269
dc.identifier.urihttp://hdl.handle.net/11023/2272
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectNeuroscience
dc.subjectOncology
dc.subject.classificationglioblastomaen_US
dc.subject.classificationp53en_US
dc.subject.classificationPDGFen_US
dc.subject.classificationSVZen_US
dc.subject.classificationPDGF-AAen_US
dc.titleCharacterization of a PDGF-AA Initiated Model of Glioblastoma with Predictable Onset
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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