Intercellular Gap Junction Communication in the Bovine Annulus Fibrosus

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dc.contributor.advisorDuncan, Neil A.
dc.contributor.authorMcWhae, Russell
dc.contributor.committeememberMatyas, John Robert
dc.contributor.committeememberSen, Arindom
dc.contributor.committeememberLi, Leping
dc.contributor.committeememberSalo, Paul T.
dc.date2019-06
dc.date.accessioned2019-02-25T15:43:56Z
dc.date.available2019-02-25T15:43:56Z
dc.date.issued2019-02-21
dc.description.abstractThe intervertebral disc has a complex, anisotropic structure. The annulus fibrosus, the fibrous outer layer of the intervertebral disc, consists of fifteen to twenty-five concentric layers of collagen fibers at alternating orientations. Cells inside and between these lamellae are known to communicate with each other through gap junctions, protein channels that directly couple the membranes of adjacent cells and form interconnected networks that may be used to coordinate a response to mechanical stimuli. These fibroblastic cells fall into three distinct morphologies: spindle-shaped lamellar cells, round lamellar cells, and interlamellar cells. With confocal microscopy methods, gap-junctional intercellular signal propagation between groups of interconnected cells was examined. While the anisotropic microenvironment of the outer annulus was hypothesized to manifest in non-homogenous signal-propagation patterns, it was demonstrated that no clear directional biases or non-homogenous behavior existed among different cell morphologies and orientations; instead, intercellular signal propagation appears to be primarily proximity based.en_US
dc.identifier.citationMcWhae, R. (2019). Intercellular Gap Junction Communication in the Bovine Annulus Fibrosus (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/36148
dc.identifier.urihttp://hdl.handle.net/1880/109915
dc.language.isoenen_US
dc.publisher.facultySchulich School of Engineeringen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectintervertebral discen_US
dc.subjectgap junctionsen_US
dc.subjectintercellular communicationen_US
dc.subjectconfocal microscopyen_US
dc.subjectbovine modelen_US
dc.subjectfluorescence loss in photobleachingen_US
dc.subject.classificationPhysiologyen_US
dc.subject.classificationHealth Sciencesen_US
dc.titleIntercellular Gap Junction Communication in the Bovine Annulus Fibrosusen_US
dc.typemaster thesisen_US
thesis.degree.disciplineEngineering – Biomedicalen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
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