Donor site wounds arise from the extraction of healthy skin for the purpose of skin grafting, employed to address extensive skin loss resulting from deep burns, ulcers, or trauma. Typically, the recovery period for these wounds spans 10-14 days, although individual variations exist, influenced by factors such as the patient's age and nutritional well-being. Post-harvesting, donor site wounds commonly exhibit increased pain levels, attributed to the exposure of dermal nerve endings. Additionally, these wounds are susceptible to infection, scar formation, and functional impairment. Although research indicates that moist dressings offer the best conditions for healing donor site wounds, the currently available dressings often fail to create an optimal environment to reduce pain and speed up recovery. This study aims to develop a bio-based moist dressing designed to manage moisture levels while offering the capability for controlled, localized drug release. Addressing the main limitations associated with the existing dressings—inefficient moisture and pain management— and aiming for immediate and tunable wound coverage, a photocrosslinkable hydrogel dressing was designed. A cellulose derivative was chosen and modified with methacrylate groups to achieve photocrosslinking. The modification process was fine-tuned by adjusting pH and time for optimal methacrylation degree. The modified cellulose was then added to a methacrylated chitosan base to form an interpenetrating polymer network. The mechanical and physical properties were evaluated for each composition of the hydrogel. Additionally, the biocompatibility, antimicrobial, antifouling and hemocompatibility effect of the best performing hydrogel were assessed. Firstly, we successfully modified the cellulose and chitosan components, leading to the formation of a stable and fast crosslinking hydrogel within 1 min. The NMR results indicated a 30% methacrylation degree for the cellulose derivative, outperforming the previously reported values. The tensile strength, rheology, and swelling ratio of the hydrogels were tuneable by adjusting the cellulose concentration in the hybrid hydrogel. Furthermore, the final hydrogel showed excellent antifouling, cell viability and hemocompatibility. In conclusion, our rapid crosslinking hydrogel, derived from modified cellulose and chitosan, addressed critical aspects of donor site wound care. With tuneable properties and proven attributes supporting wound management, it promises advancement in donor site wound treatment.