Studies of mouse cerebellar compartmentation

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dc.contributor.advisorHawkes, Richard
dc.contributor.authorChung, Seunghyuk
dc.date.accessioned2017-12-18T21:44:04Z
dc.date.available2017-12-18T21:44:04Z
dc.date.issued2008
dc.descriptionBibliography: p. 321-386en
dc.description.abstractThe cerebellum is an appealing model system for studying pattern formation. Despite its uniform histology, there is a complex underlying pattern of parasagittal stripes involving multiple cell types. This patterning is thought to be a highly conserved feature both during development and in the adult. To explore this issue, I first present that the expression of gamma-aminobutyric acid B receptor 2 is in an array of Purkinje cell stripes identical to that revealed by anti-zebrin II, which is the most studied cerebellar compartmentation antigen. Secondly, I present expression data showing that the pattern of phospholipase CB4 expression, a zebrin II-imrnunonegative Purkinje cell marker, can be traced continuously from the embryo to the adult. Thirdly, I describe a gene, Early B­cell factor 2 (EBF2) that suppresses the zebrin II-imrnunopositive phenotype. EBF2 expression is confined to the zebrin II-imrnunonegative population and zebrin II is uniformly expressed in the Ebj2 null cerebellum due to a combination of selective Purkinje cell death and ectopic expression of genes normally restricted to the zebrin II­imrnunonegative Purkinje cell subset. Fourthly, I investigated the topographical relationship between zebrin II compartmentation and the distribution of unipolar brush cells. The data suggest that unipolar brush cell somata become regionally restricted through interactions with specific Purkinje cell subtypes. Finally, the cerebellar nuclei (CN) are the next relay stage in the cerebellar circuit and receive the sole output from Purkinje cells. However, there is no molecular complexity in the CN equivalent to that in the cerebellar cortex. To begin to address this gap, I first built an adult CN map by using a novel marker - kinesin light chain 3. Then, I combined the use of various antigens and transgenes to reveal a complex heterogeneity in the mouse CN that can be used to subdivide the CN into twelve reproducible expression domains. Similar heterogeneity can also be revealed during development: the medial nuclei are constructed from two separate neuronal subsets, each with a distinct embryological origin. These data suggest that a complex topography similar to that seen in the cerebellar cortex, is also present in the CN.
dc.format.extentxxii, 386 leaves : ill. ; 30 cm.en
dc.identifier.citationChung, S. (2008). Studies of mouse cerebellar compartmentation (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/2265en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/2265
dc.identifier.urihttp://hdl.handle.net/1880/103266
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleStudies of mouse cerebellar compartmentation
dc.typedoctoral thesis
thesis.degree.disciplineNeuroscience
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1779 520708942
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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