Characterization of Neutralizing and Non-neutralizing Epitopes of Porcine Circovirus 2 Capsid Protein

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atmire.migration.oldid3371
dc.contributor.advisorCzub, Markus
dc.contributor.authorNourozieh, Narges
dc.date.accessioned2015-07-09T19:07:04Z
dc.date.available2015-11-20T08:00:31Z
dc.date.issued2015-07-09
dc.date.submitted2015en
dc.description.abstractVaccination is the most efficacious way to prevent Porcine circovirus 2 (PCV2)-associated diseases. In experimental pig studies, vaccine-induced neutralizing antibodies (NAs) appear to play a major role in protection from PCV2 infection. The immune response to PCV2 vaccination of farmed pigs has not been studied in detail. I hypothesize that NAs target conformational epitope(s) present on the surface of PCV2 particles. Highly purified PCV2 particles were found to expose only conformational but not linear epitopes on their surface. The screening 160 sera from farmed pigs showed that reactivity of sera with PCV2 particles correlated positively with the level of NA titer, suggesting that NAs recognize surface-exposed conformational epitope(s). This finding was supported by depleting antibodies reacting with linear epitopes of PCV2. No significant change was observed in the level of NA titer after antibody depletion. Altogether, these data suggest that NAs target conformational epitope(s) on the surface of PCV2 particles.en_US
dc.identifier.citationNourozieh, N. (2015). Characterization of Neutralizing and Non-neutralizing Epitopes of Porcine Circovirus 2 Capsid Protein (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25278en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25278
dc.identifier.urihttp://hdl.handle.net/11023/2337
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectVeterinary Science
dc.subjectVirology
dc.subjectImmunology
dc.subject.classificationPorcine circovirus 2 (PCV2)en_US
dc.subject.classificationConformational epitopeen_US
dc.subject.classificationNeutralizing antibodyen_US
dc.titleCharacterization of Neutralizing and Non-neutralizing Epitopes of Porcine Circovirus 2 Capsid Protein
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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