The Effects of Smoking on Autophagy in Transformed Cell Lines and Monocyte-derived Dendritic Cells of NOD2 Genotyped Inflammatory Bowel Disease Patients

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atmire.migration.oldid4407
dc.contributor.advisorGhosh, Subrata
dc.contributor.authorchenoo, Shem
dc.contributor.committeememberBeck, Paul
dc.contributor.committeememberKaplan, Gilaad
dc.contributor.committeememberHirota, Simon
dc.date.accessioned2016-05-06T15:41:10Z
dc.date.available2016-05-06T15:41:10Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractGenome-wide association studies have identified around 200 genes associated with inflammatory bowel disease (IBD) among which 3 susceptible genes including NOD2, ATG16L1 and IRGM are found to increase risk of Crohn’s Disease (CD) and altering the metabolic pathway known as autophagy. The aim of this study is to look at how smoking which is detrimental in CD but beneficial to ulcerative colitis (UC) affects autophagy in both cell lines as well as primary cells. HeLa cells and THP-1 like macrophages as well as monocyte-derived dendritic cells were treated with cigarette smoke extract (CSE) which is a surrogate of smoking at different concentrations for 24h. Cells were treated with rapamycin or muramyl dipeptide (MDP) and autophagy was assessed by microscopy and Western blotting. CSE significantly inhibits autophagy in HeLa cells and THP-1 like macrophages through both the mTOR and NOD2 mediated autophagic pathways.en_US
dc.identifier.citationchenoo, S. (2016). The Effects of Smoking on Autophagy in Transformed Cell Lines and Monocyte-derived Dendritic Cells of NOD2 Genotyped Inflammatory Bowel Disease Patients (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28118en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28118
dc.identifier.urihttp://hdl.handle.net/11023/2990
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectImmunology
dc.titleThe Effects of Smoking on Autophagy in Transformed Cell Lines and Monocyte-derived Dendritic Cells of NOD2 Genotyped Inflammatory Bowel Disease Patients
dc.typemaster thesis
thesis.degree.disciplineGastrointestinal Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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