Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani
| dc.contributor.author | McNeil, Bonnie A | |
| dc.contributor.author | Simon, Dawn M | |
| dc.contributor.author | Zimmerly, Steven | |
| dc.date.accessioned | 2016-04-15T22:41:43Z | |
| dc.date.available | 2016-04-15T22:41:43Z | |
| dc.date.issued | 2013-10 | |
| dc.description.abstract | Group II introns are ribozymes and retroelements found in bacteria, and are thought to have been the ancestors of nuclear pre-mRNA introns. Whereas nuclear introns undergo prolific alternative splicing in some species, group II introns are not known to carry out equivalent reactions. Here we report a group II intron in the human pathogen Clostridium tetani, which undergoes four alternative splicing reactions in vivo. Together with unspliced transcript, five mRNAs are produced, each encoding a distinct surface layer protein isoform. Correct fusion of exon reading frames requires a shifted 5' splice site located 8 nt upstream of the canonical boundary motif. The shifted junction is accomplished by an altered IBS1-EBS1 pairing between the intron and 5' exon. Growth of C. tetani under a variety of conditions did not result in large changes in alternative splicing levels, raising the possibility that alternative splicing is constitutive. This work demonstrates a novel type of gene organization and regulation in bacteria, and provides an additional parallel between group II and nuclear pre-mRNA introns. | en_US |
| dc.description.refereed | Yes | en_US |
| dc.identifier.citation | McNeil, B.A., Simon, D.M., Zimmerly, S. (2014) Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani. Nucleic Acids Research 42:1959-1969. doi: 10.1093/nar/gkt1053 | en_US |
| dc.identifier.doi | 10.1093/nar/gkt1053 | |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/35157 | |
| dc.identifier.uri | http://hdl.handle.net/1880/51152 | |
| dc.language.iso | en | en_US |
| dc.publisher | Oxford Journals | en_US |
| dc.publisher.department | Biological Sciences | en_US |
| dc.publisher.faculty | Science | en_US |
| dc.publisher.institution | University of Calgary | en_US |
| dc.publisher.url | http://nar.oxfordjournals.org/content/early/2013/11/07/nar.gkt1053.full.pdf+html | en_US |
| dc.rights | Attribution 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.title | Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani | en_US |
| dc.type | journal article |