The Effect of Myostatin on Glucose Metabolism and Insulin Sensitivity

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atmire.migration.oldid790
dc.contributor.advisorHittel, Dustin
dc.contributor.authorGhozlan, Mostafa
dc.date.accessioned2013-04-05T22:01:54Z
dc.date.available2013-06-15T07:01:51Z
dc.date.issued2013-04-05
dc.date.submitted2013en
dc.description.abstractAim : Myostatin (MSTN) is a negative regulator of skeletal muscle proliferation and differentiation that we previously associated with insulin resistance in humans and mice. We assessed its potential regulatory role in glucose metabolism and insulin sensitivity in vivo and in vitro. Methods: Using various approaches we assessed the following parameters: in vivo GTT and ITT and in vitro insulin-stimulated glucose uptake for insulin sensitivity, qRT-PCR for Glut1, Glut4, Gyg1, Hk2, IL-6, Western blotting for protein levels of AMPK and ACC and high-resolution respirometry for glucose-dependent mitochondrial respiration. Results: MSTN showed significant reduction of hepatic insulin-stimulated glucose uptake in vitro and reduced glycogen content in vivo without affecting glycolysis, glucose metabolism genes, AMPK signalling or mitochondrial respiration. Conclusion: This study presents evidence for the potential role of liver in MSTN-induced insulin resistance; especially glucose uptake and glycogen content, which necessitates further investigation of the role of MSTN in this metabolically critical organ.en_US
dc.identifier.citationGhozlan, M. (2013). The Effect of Myostatin on Glucose Metabolism and Insulin Sensitivity (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28087en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28087
dc.identifier.urihttp://hdl.handle.net/11023/588
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Molecular
dc.subjectBiochemistry
dc.subject.classificationMyostatinen_US
dc.subject.classificationGlucoseen_US
dc.subject.classificationInsulinen_US
dc.subject.classificationSensitivityen_US
dc.subject.classificationMuscleen_US
dc.subject.classificationLiveren_US
dc.subject.classificationGlycogenen_US
dc.subject.classificationMitochondriaen_US
dc.subject.classificationRespirometryen_US
dc.subject.classificationMetabolismen_US
dc.titleThe Effect of Myostatin on Glucose Metabolism and Insulin Sensitivity
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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