Chloride Dynamics and the Control of CRH Activity

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dc.contributor.advisorBains, Jaideep
dc.contributor.advisorGordon, Grant Robert J.
dc.contributor.authorLanz, Aaron Justin
dc.contributor.committeememberThompson, Roger J.
dc.contributor.committeememberEpp, Jonathan Richard
dc.date2019-11
dc.date.accessioned2019-07-09T13:47:22Z
dc.date.available2019-07-09T13:47:22Z
dc.date.issued2019-07-05
dc.description.abstractCRH neurons of the PVN release the neuropeptide CRH to initiate the endocrine stress response and to modulate local glutamatergic synapses. The cellular mechanisms that promote CRH release during an acute stress are unknown. Here we show that CRH neurons begin to burst following an acute stress in vitro. Using cell-attached recordings and a novel optogenetic strategy, we demonstrate that KCC2 is suppressed following an acute stress and that this functional downregulation is sufficient to promote bursting in CRH neurons. Further, we show that KCC2 suppression leads to local CRH release in the PVN to promote short-term plasticity at glutamate synapses. This study offers a new tool to study chloride homeostasis in brain slices and provides evidence that KCC2 activity dynamics can alter cell spiking and synaptic plasticity in the healthy brain.en_US
dc.identifier.citationLanz, A. J. (2019). Chloride Dynamics and the Control of CRH Activity (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/36717
dc.identifier.urihttp://hdl.handle.net/1880/110602
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectBurstingen_US
dc.subjectChloride homeostasisen_US
dc.subjectStressen_US
dc.subject.classificationNeuroscienceen_US
dc.titleChloride Dynamics and the Control of CRH Activityen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Neuroscienceen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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