Methane Cycling and Methanotrophic Bacteria in Base Mine Lake, a Model End-Pit Lake in the Alberta Oilsands

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dc.contributor.advisorDunfield, Peter F.
dc.contributor.authorAlbakistani, Emad
dc.contributor.committeememberVoordouw, Gerrit
dc.contributor.committeememberGieg, Lisa Marie
dc.date2018-11
dc.date.accessioned2018-09-05T14:35:53Z
dc.date.available2018-09-05T14:35:53Z
dc.date.issued2018-08-29
dc.description.abstractWe studied methanotrophic bacteria over three years (2015 - 2018) in Base Mine Lake, Fort McMurray, Canada. The lake represents the first large scale demonstration of end-pit lake technology in the Alberta oilsands. 16S rRNA gene amplicon sequencing and measurement of methanotrophic rates were applied to evaluate the effect of seasonal changes on methanotrophic diversity and activity, and to understand the biogeochemical cycling of methane and oxygen. Based on 16S rRNA gene sequence relative abundance, the predominantly detected methanotrophic genera were Methylobacter/Crenothrix in the winter and Methylocaldum in the summer. Methanotrophs were most abundant in winter throughout the water column, and in summer at the bottom of the lake near the fluid fine tailings interface. Potential methanotrophic rates decreased over three years from 2015-2018.en_US
dc.identifier.citationAlbakistani, E. (2018). Methane Cycling and Methanotrophic Bacteria in Base Mine Lake, a Model End-Pit Lake in the Alberta Oilsands (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32877en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/32877
dc.identifier.urihttp://hdl.handle.net/1880/107699
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyScience
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject.classificationMicrobiologyen_US
dc.subject.classificationEnvironmental Sciencesen_US
dc.titleMethane Cycling and Methanotrophic Bacteria in Base Mine Lake, a Model End-Pit Lake in the Alberta Oilsands
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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