Biophysical Characterization of Tear Film Biomimetics: Interactions of Major Polar Lipids and Proteins

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atmire.migration.oldid6202
dc.contributor.advisorPrenner, Elmar
dc.contributor.authorHaley, David Alan
dc.contributor.committeememberNoskov, Sergei
dc.contributor.committeememberVogel, Hans
dc.date.accessioned2017-11-30T21:10:08Z
dc.date.available2017-11-30T21:10:08Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractThe tear film is a multilayered structure that protects the corneal epithelium from stress and damage, and allows for clear vision. A lipid layer shields the primarily aqueous portion of the tear film from the external environment. A polar lipid layer provides an interface between the aqueous layer and a thick nonpolar lipid layer. Dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, palmitoyl-glucocerebroside, palmitoylsphingomyelin, and dipalmitoylphosphatidylserine have been previously identified as polar lipids in whole tear samples. Langmuir trough monolayer experiments were used to assess how major tear film proteins lysozyme, lactoferrin, and tear lipocalin affect the stability and elasticity of the pure lipids and lipid mixtures. Pure lipids and lipid mixtures all displayed high stability and rigidity in the tear film surface pressure range of 20 to 35 mN/m. Biologically relevant concentrations of lysozyme and lactoferrin (> 0.2 mg/mL) appeared to fluidize a quinary lipid mixture. Low concentrations of lipocalin may destabilize polar lipid films.en_US
dc.identifier.citationHaley, D. A. (2017). Biophysical Characterization of Tear Film Biomimetics: Interactions of Major Polar Lipids and Proteins (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25136en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25136
dc.identifier.urihttp://hdl.handle.net/11023/4258
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology
dc.subjectBiology--Molecular
dc.subjectBiophysics
dc.subjectBiochemistry
dc.subject.otherdipalmitoylphosphatidylcholine
dc.subject.otherdipalmitoylphosphatidylethanolamine
dc.subject.otherpalmitoylglucocerebroside
dc.subject.otherpalmitoylsphingomyelin
dc.subject.otherdipalmitoylphosphatidylserine
dc.subject.otherDPPC
dc.subject.otherDPPE
dc.subject.otherPSM
dc.subject.otherPGC
dc.subject.otherDPPS
dc.subject.othertear film
dc.subject.otherlipid
dc.subject.otherLangmuir
dc.subject.otherBAM
dc.subject.otherBrewster Angle Microscopy
dc.subject.othersurface pressure
dc.subject.othereye
dc.subject.otherProtein
dc.subject.otherlysozyme
dc.subject.otherlactoferrin
dc.subject.othertear lipocalin
dc.subject.othermonolayer
dc.titleBiophysical Characterization of Tear Film Biomimetics: Interactions of Major Polar Lipids and Proteins
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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