Microtubules and fertilization: The MEI-1/Katanin mediated cytoskeletal transition from meiosis to mitosis in the developing C. elegans embryo
| atmire.migration.oldid | 1843 | |
| dc.contributor.advisor | Mains, Paul | |
| dc.contributor.author | Beard, Sarah Marie | |
| dc.date.accessioned | 2014-01-28T20:54:10Z | |
| dc.date.available | 2014-03-15T07:00:19Z | |
| dc.date.issued | 2014-01-28 | |
| dc.date.submitted | 2014 | en |
| dc.description.abstract | During embryonic development, dramatic changes of the C. elegans cytoskeleton occur in the transition from the meiosis to mitosis requiring precise regulation of molecules specific to each type of spindle. The microtubule severing complex, MEI-1/MEI-2, is required for C. elegans meiotic spindle formation, but must be inactivated to prevent disruption of mitosis. There are several redundant pathways involved in this tightly regulated process. In order to determine how known genes of these pathways function relative to one another, I have developed a robust antibody-staining assay to measure anti-MEI-1 protein levels based on total pixel intensity in early embryogenesis. Using this assay, I have shown that APC and MBK-2 act strictly sequentially with one another to degrade MEI-1. I have also shown that ectopic MEI-1 staining in cul-2 and rfl-1 are enhanced by mel-26 and not by mbk-2 indicating that CUL-2 and MBK-2 are working together to degrade MEI-1 in parallel to the MEL-26/CUL-2 pathway and that RFL-1 is activating CUL-2 in this process. hecd-1 ectopic MEI-1 staining was not enhanced by any of the known regulatory genes in this process and might be inhibiting an activator of MEI-1. This project will assist in decoding the key regulatory molecules of the developmental remodelling in early embryos. | en_US |
| dc.identifier.citation | Beard, S. M. (2014). Microtubules and fertilization: The MEI-1/Katanin mediated cytoskeletal transition from meiosis to mitosis in the developing C. elegans embryo (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26912 | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/26912 | |
| dc.identifier.uri | http://hdl.handle.net/11023/1303 | |
| dc.language.iso | eng | |
| dc.publisher.faculty | Graduate Studies | |
| dc.publisher.institution | University of Calgary | en |
| dc.publisher.place | Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | |
| dc.subject | Genetics | |
| dc.subject.classification | MEI-1 | en_US |
| dc.subject.classification | Katanin | en_US |
| dc.subject.classification | Mitosis | en_US |
| dc.subject.classification | Meiosis | en_US |
| dc.subject.classification | Microtubules | en_US |
| dc.title | Microtubules and fertilization: The MEI-1/Katanin mediated cytoskeletal transition from meiosis to mitosis in the developing C. elegans embryo | |
| dc.type | master thesis | |
| thesis.degree.discipline | Medical Science | |
| thesis.degree.grantor | University of Calgary | |
| thesis.degree.name | Master of Science (MSc) | |
| ucalgary.item.requestcopy | true |