Comparative innate responses induced by Toll-like receptor (TLR)7 and 21 ligands against infectious bronchitis virus infection

De Silva Senapathi, Yaseshwari Upasama

Comparative innate responses induced by Toll-like receptor (TLR)7 and 21 ligands against infectious bronchitis virus infection

dc.contributor.advisor	Abdul-Careem, Mohamed Faizal
dc.contributor.author	De Silva Senapathi, Yaseshwari Upasama
dc.contributor.committeemember	Czub, Markus
dc.contributor.committeemember	Van Marle, Guido
dc.date	2019-06
dc.date.accessioned	2019-05-02T15:41:27Z
dc.date.available	2019-05-02T15:41:27Z
dc.date.issued	2019-01-26
dc.description.abstract	Toll-like receptor (TLR)7 and 21 ligands, resiquimod and cytosine-guanosine (CpG) oligonucleotides (ODNs) respectively have been used in ovo (pre-hatch) to enhance or prime an early immune response in chickens to provide protection against microbial pathogens. Yet, their protective efficacy against an infectious bronchitis virus (IBV) infection encountered post-hatch has not been evaluated. Thus, our objectives were to investigate the efficacy of resiquimod and CpG ODNs against a post-hatch IBV infection by delivering the ligands in ovo at embryo day (ED)18 and then, to determine possible mechanisms of protection. We found upregulation of interleukin (IL)-1β and interferon (IFN)-γ mRNA levels and considerable expansions of macrophage and cluster of differentiation (CD)8α+ T cell populations in lungs of chicken as early as day one post-hatch, following pre-hatch delivery of resiquimod. When the resiquimod pre-treated day-old chickens were infected with IBV, reduction in viral shedding via oral and fecal routes was observed at 3 days post-infection (dpi). Similarly, in CpG ODN pre-treated birds at 3 dpi, we found increased recruitment of macrophages, CD8α+ and CD4+ T lymphocytes in addition to up-regulation of IFN-γ and IL-1β mRNA concentrations in chicken lungs. However, only in ovo delivered CpG ODNs significantly reduced the morbidity and mortality associated with IBV infection. Overall, these studies bring us closer to understanding mechanisms behind CpG ODNs and resiquimod induced immune responses in chickens when used as stand-alone prophylactic agents in ovo.	en_US
dc.identifier.citation	De Silva Senapathi, Y. U. (2019). Comparative innate responses induced by Toll-like receptor (TLR)7 and 21 ligands against infectious bronchitis virus infection (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/36426
dc.identifier.uri	http://hdl.handle.net/1880/110244
dc.language.iso	eng	en_US
dc.publisher.faculty	Veterinary Medicine	en_US
dc.publisher.institution	University of Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.	en_US
dc.subject	In ovo; CpG oligonucleotide; resiquimod, infectious bronchitis virus; avian macrophage; CD4+ cell; CD8α+ cell; interleukin-1β; interferon-γ	en_US
dc.subject.classification	Veterinary Science	en_US
dc.subject.classification	Virology	en_US
dc.subject.classification	Immunology	en_US
dc.title	Comparative innate responses induced by Toll-like receptor (TLR)7 and 21 ligands against infectious bronchitis virus infection	en_US
dc.type	master thesis	en_US
thesis.degree.discipline	Veterinary Medical Sciences	en_US
thesis.degree.grantor	University of Calgary	en_US
thesis.degree.name	Master of Science (MSc)	en_US
ucalgary.item.requestcopy	true