Construction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResT

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atmire.migration.oldid1599
dc.contributor.advisorChaconas, George
dc.contributor.authorBandy, Nick
dc.date.accessioned2013-10-09T16:25:06Z
dc.date.available2013-11-12T08:00:21Z
dc.date.issued2013-10-09
dc.date.submitted2013en
dc.description.abstractDuring DNA replication in Borrelia burgdorferi, replication of linear elements results in an inverted dimer repeat of the telomere which is subsequently resolved by the telomere resolvase, ResT. ResT has been demonstrated as essential and a knock-out strain has never been produced. Using a conditional expression system a resT knock-out strain has been produced and used to investigate the effects of ResT depletion of spirochete viability as well as genome stability. ResT depleted spirochetes ceased to divide after ResT depletion, however, remained viable up to 16 days suggesting an arrested state of the spirochetes. Southern blots of the DNA revealed a shift of linear plasmid DNA to a higher molecular weight representing the circular intermediate. DNA replication did not continue in spirochetes after ResT depletion suggesting ResT may play a larger role in replication than previously thought.en_US
dc.identifier.citationBandy, N. (2013). Construction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResT (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25430en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25430
dc.identifier.urihttp://hdl.handle.net/11023/1139
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGenetics
dc.subjectMicrobiology
dc.subjectBiochemistry
dc.subject.classificationBorreliaen_US
dc.subject.classificationTelomereen_US
dc.subject.classificationLymeen_US
dc.titleConstruction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResT
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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