Magnetic Resonance Imaging for Tracking of Cells and Agents Targeted to Bone Fracture

Taha, May

Magnetic Resonance Imaging for Tracking of Cells and Agents Targeted to Bone Fracture

atmire.migration.oldid	3119
dc.contributor.advisor	Dunn, Jeff
dc.contributor.advisor	Matyas, John
dc.contributor.author	Taha, May
dc.date.accessioned	2015-04-29T19:13:31Z
dc.date.available	2015-06-22T07:00:47Z
dc.date.issued	2015-04-29
dc.date.submitted	2015	en
dc.description.abstract	Regenerative medicine is likely to play a major role in the treatment of musculoskeletal diseases. Stem cell therapies could be used to restore damaged or diseased tissues by contributing to the healing process. Additionally, bone targeted nanoparticles could be of great use in this field, as they can be used to deliver therapies or to image an injury site. In order to evaluate and monitor new regenerative therapies in preclinical models over time, a non-invasive in vivo imaging tool is needed. Use of such an imaging method will enable testing new cell therapies in bone. Magnetic resonance imaging (MRI) holds considerable promise for this purpose. Considering that it is a non-invasive and non ionizing method makes it well-suited for repeated measurements studies. Initially, we optimized an MRI protocol for visualization of bone injuries, and then we compared the optimized MRI protocol with µCT as the gold standard for bone imaging. We found that MRI offers several advantages over µCT, including that it visualized soft tissue, edema, therapeutic biomaterials, and is especially useful when ionizing radiation is to be avoided. Subsequently, we used MRI to assess materials used in this study such as bone targeted nanoparticles, contrast agents and stem cell scaffolds. After establishing the MRI protocol, we labelled-differentiated ESCs, and then transplanted them in vivo for MR tracking. The results showed that MRI detected the labelled cells in vivo that under some conditions the MRI could detect migration of the differentiated ESC’s to remote site of injury. The findings were validated by histology and immunohistochemistry. To our knowledge, this is the first study to track cells in bone fracture using MRI. Based on the results of this research, future studies can use the developed cell tracking model for testing the effectiveness of novel cell therapies that promote bone repair.	en_US
dc.identifier.citation	Taha, M. (2015). Magnetic Resonance Imaging for Tracking of Cells and Agents Targeted to Bone Fracture (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26810	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/26810
dc.identifier.uri	http://hdl.handle.net/11023/2174
dc.language.iso	eng
dc.publisher.faculty	Graduate Studies
dc.publisher.institution	University of Calgary	en
dc.publisher.place	Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject	Medicine and Surgery
dc.subject.classification	Magnetic Resonance Imaging	en_US
dc.subject.classification	Micro Computed Tomography	en_US
dc.subject.classification	Stem Cells	en_US
dc.subject.classification	Cell labelling	en_US
dc.subject.classification	Cell tracking	en_US
dc.subject.classification	Bone	en_US
dc.subject.classification	Musculoskeletal system	en_US
dc.subject.classification	Contrast agents	en_US
dc.title	Magnetic Resonance Imaging for Tracking of Cells and Agents Targeted to Bone Fracture
dc.type	doctoral thesis
thesis.degree.discipline	Medical Science
thesis.degree.grantor	University of Calgary
thesis.degree.name	Doctor of Philosophy (PhD)
ucalgary.item.requestcopy	true