Inner Calcium Stores and P2 Receptors are Involved in Regulating Reactivated Sperm Motility

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atmire.migration.oldid4894
dc.contributor.advisorVan Der Hoorn, Franciscus
dc.contributor.authorHamed, Akram
dc.contributor.committeememberDobrinski, Ina
dc.contributor.committeememberThundathil, Jacob
dc.contributor.committeememberChen, Wayne
dc.date.accessioned2016-09-13T18:28:46Z
dc.date.available2016-09-13T18:28:46Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractWe used a sperm reactivation model to study the possible role of the sperm calcium inner stores in regulating sperm motility reactivation. Our results show that the release of calcium from the stores could be important for sperm motility reactivation. In addition, stimulating the release of calcium from the stores using drugs like thimerosal resulted in a significant change in motility pattern of reactivated sperm. Moreover, our immunofluorescence results showed—for the first time—the expression and localization of the two P2Y receptors, P2Y1 and P2Y2, and the P2X7 receptor on the sperm tail of mouse sperm. Inhibiting P2 receptors resulted in inhibition of sperm motility reactivation suggesting the possible role of the receptors in motility reactivation. In conclusion, the possible importance of the calcium inner stores and the P2 receptors in motility reactivation suggests that they might have a role in regulating sperm functions.en_US
dc.identifier.citationHamed, A. (2016). Inner Calcium Stores and P2 Receptors are Involved in Regulating Reactivated Sperm Motility (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26536en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26536
dc.identifier.urihttp://hdl.handle.net/11023/3294
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiochemistry
dc.titleInner Calcium Stores and P2 Receptors are Involved in Regulating Reactivated Sperm Motility
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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