Interactions between Serum Albumin Proteins and Polystyrene Nanoparticles

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dc.contributor.advisorCramb, David Thomas
dc.contributor.authorBishop, Amanda Iris
dc.contributor.committeememberHeyne, Belinda J. M.
dc.contributor.committeememberLing, Chang-Chun
dc.contributor.committeememberHarrison, Joe J.
dc.date2018-11
dc.date.accessioned2018-07-12T18:52:16Z
dc.date.available2018-07-12T18:52:16Z
dc.date.issued2018-07-10
dc.description.abstractNanoparticles (NPs) have become increasingly popular for several applications, especially regarding biomedical applications because of their unique properties. However, when a nanoparticle enters a biological medium, it is thought to become encapsulated in proteins and other biomolecules in a coating termed a “protein corona.” This coating is significant as it can change the identity and surface properties of the nanoparticle, thus affecting its fate within the biological medium. Studies on the formation of NP-protein complexes have been ongoing for years although the interactions are still not fully understood due to their dynamics and complexity. As a result, this hinders the use of NPs to their full potential in biomedical applications. The studies performed in this thesis analyze the interactions of fluorescent polystyrene nanoparticles (FS) of two different sizes with bovine serum albumin proteins (BSA) by the technique of Two-Photon Excitation Cross-correlation Spectroscopy (TPE-FCCS). These interactions were explored both thermodynamically and kinetically to gain insight into the formation of the early hard corona and the kinetics of the formation of the BSA-FS complexes. The results suggested very low binding ratios and a mechanism of protein association dependent on the size of the sphere present in solution. The results also suggested an irreversible formation of BSA-FS complexes, in which the BSA appears to stack at the surface of the FS. These findings are significant as they challenge the current beliefs on the formation of a protein corona and perceived monolayer formation, and furthermore, provides a deeper understanding of NP-protein interactions.en_US
dc.identifier.citationBishop, A. I. (2018). Interactions between Serum Albumin Proteins and Polystyrene Nanoparticles (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32363en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/32363
dc.identifier.urihttp://hdl.handle.net/1880/107141
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyScience
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectNanoparticles
dc.subjectserum albumin proteins
dc.subjectpolystyrene nanoparticles
dc.subjectprotein corona
dc.subjecttwo-photon excitation
dc.subject.classificationEducation--Sciencesen_US
dc.titleInteractions between Serum Albumin Proteins and Polystyrene Nanoparticles
dc.typemaster thesis
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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