Biochemical characterization of Shewanella-like protein phosphatase 1 in Arabidopsis thaliana

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dc.contributor.advisorMoorhead, Greg
dc.contributor.authorVahab, Ahmad Reza
dc.contributor.committeememberRo, dae kyun
dc.contributor.committeememberNg, Kenneth
dc.date2018-06
dc.date.accessioned2018-01-22T23:55:14Z
dc.date.available2018-01-22T23:55:14Z
dc.date.issued2018-01-09
dc.description.abstractThe Arabidopsis thaliana genome encodes two isoforms of Shewanella-like phosphatases - SLP1, which is localized in chloroplasts of photosynthetic tissues, and SLP2, a protein localized in mitochondria consistent with its origin in bacteria. Due to its unique pattern of chloroplast localization, characterization of SLP1 was undertaken to identify its substrates and establish its biological function. In the presented work, one of the largest quantitative phosphoproteomic studies on A. thaliana was conducted to identify SLP1's potential substrates by comparing WT and slp1 KO phosphoproteomic profiles. This led to identification of 170 high-confidence substrates, all residing in chloroplast and may be directly involved in SLP1 signaling network, including starch synthesis regulation and protein import into chloroplasts. A close examination of phospho-peptides from the identified substrates revealed a unique acidic motif surrounding the SLP1 (de)phosphorylation sites, resembling the chloroplastic casein kinase II (cpCKII) phosphorylation sites.en_US
dc.identifier.citationVahab, A.R. (2018). Biochemical characterization of Shewanella-like protein phosphatase 1 in Arabidopsis thaliana. University of Calgary, Calgary, ABen_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/5384
dc.identifier.urihttp://hdl.handle.net/1880/106303
dc.language.isoenen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectBacterial-like phosphataseen_US
dc.subjectArabidopsis thalianaen_US
dc.subjectSLP1en_US
dc.subjectAtSLP1en_US
dc.subjectPhosphorylationen_US
dc.subjectQuantitative phosphoproteomicsen_US
dc.subjectPlant biochemistryen_US
dc.subjectShewanella-likeen_US
dc.subjectStarch metabolism regulationen_US
dc.subjectSS1en_US
dc.subjectStarch syntahse 1en_US
dc.subjectProtein import phosphorylation regulationen_US
dc.subject.classificationEducation--Sciencesen_US
dc.subject.classificationBiochemistryen_US
dc.titleBiochemical characterization of Shewanella-like protein phosphatase 1 in Arabidopsis thalianaen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
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