Metabolomics Study of the Diagnosis and Prognosis of Severe Traumatic Brain Injury (sTBI)

Simple item page
dc.contributor.advisorWinston, Brent W
dc.contributor.authorBanoei, Mohammad Mehdi
dc.contributor.committeememberHabibi, Hamid R
dc.contributor.committeememberLewis, Ian A
dc.contributor.committeememberCouillard, Phillippe
dc.contributor.committeememberDebert, Chantel T
dc.contributor.committeememberFraser, Douglas D;
dc.dateWinter Conferral
dc.date.accessioned2022-03-14T22:27:50Z
dc.date.available2022-03-14T22:27:50Z
dc.date.issued2020-11-30
dc.description.abstractTraumatic brain injury (TBI) is defined as neurologic injury resulting from an external mechanical force, which is associated with long-term neurological and cognitive disability and affects individuals of all ages, ethnicities, and socioeconomic characteristics. TBI severe enough to cause hospitalization occurs in over 10 million people each year worldwide. TBI is the most common cause of death and long-term disability in children and young adults in developed countries. Currently, clinical assessment and neuroimaging (e.g. CT and MRI) are the most reliable techniques used for the diagnosis and prognosis of TBI. Unfortunately, this approach has considerable shortcomings when considering sensitivity and specificity of TBI diagnosis and prognosis. Disease stratification and the prediction of outcomes and are key problems for the management of severe TBI (sTBI).This study showed that metabolomics can be applied for the diagnosis and prognosis of sTBI using nuclear magnetic resonance (NMR) spectroscopy and direct infusion tandem mass spectrometry (DI-MS/MS). The results are promising for the diagnose sTBI when compared to orthopedic injury (OI) controls and for the prognostication sTBI outcomes at 3, 6, and 12-months post-injury particularly in predicting poor outcomes from the good outcome. We also investigated the metabolomics on animal models for sTBI. Using the cortical controlled impact (CCI) mouse model of sTBI demonstrated a difference in metabolic profiles of CCI and CCI mice receiving a plastic CAP to cover the skull in the craniotome area (CCI+CAP) mice when compared to sham controls. Our result revealed that the highest degree of metabolic alterations occurs at 8 hours post-injury and that the CCI+CAP mice have a prolonged brain injury compared to CCI mice (assessed at 7 days post-injury).
dc.identifier.citationBanoei, M. (2020). Metabolomics Study of the Diagnosis and Prognosis of Severe Traumatic Brain Injury (sTBI) (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/39644
dc.identifier.urihttp://hdl.handle.net/1880/114484
dc.language.isoenen
dc.language.isoEnglish
dc.publisher.facultyGraduate Studiesen
dc.publisher.facultyCumming School of Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en
dc.subjectMetabolomics
dc.subjectsevere Traumatic Brain Injury
dc.subjectBiomarkers
dc.subjectPrognosis and diagnosis of sTBI.
dc.subject.classificationHealth Sciences--Medicine and Surgery
dc.subject.classificationHealth Sciences--General
dc.titleMetabolomics Study of the Diagnosis and Prognosis of Severe Traumatic Brain Injury (sTBI)
dc.typedoctoral thesis
thesis.degree.disciplineMedicine – Medical Sciences
thesis.degree.grantorUniversity of Calgaryen
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ucalgary_2020_banoei_mohammadmehdi.pdf
Size:
12.9 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Open Theses and Dissertations