The Development and Characterization of Three-Colour Fluorescence Cross-Correlation Spectroscopy

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atmire.migration.oldid2952
dc.contributor.advisorCramb, David
dc.contributor.authorBlades, Megan
dc.date.accessioned2015-02-06T22:51:25Z
dc.date.embargolift10000-01-01
dc.date.issued2015-02-06
dc.date.submitted2015en
dc.description.abstractExpanding on theoretical work by Heinze et al.1, an optical setup to experimentally realize three-colour fluorescence cross-correlation spectroscopy (3C-FCCS) was designed. This instrument simultaneously collects and correlates fluorescence data from three colour channels, allowing real-time tracking of three-coloured species in complex solutions. To examine 3C-FCCS theory, a nanobarcode test particle, which physically linked three fluorophores was selected and characterized. These nanobarcode particles, capable of producing a triple cross-correlation decay, facilitated proof-of-concept experiments. 3C-FCCS was shown to be capable of measuring the concentration of nanobarcode particles in solution exclusively containing nanobarcode particles and solution containing nanobarcodes outnumbered by background quantum dots at a ratio of 800:1. 3C-FCCS was used to investigate a simple DNA-based nanostructure. These experiments illustrate that 3C-FCCS is a compelling technique with a plethora of potential applications, ranging from investigating hierarchical nanoassembly to biological reactions.en_US
dc.description.embargotermsindefiniteen_US
dc.identifier.citationBlades, M. (2015). The Development and Characterization of Three-Colour Fluorescence Cross-Correlation Spectroscopy (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28052en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28052
dc.identifier.urihttp://hdl.handle.net/11023/2090
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectChemistry--Physical
dc.subject.classificationFluorescence Cross-Correlation Spectroscopyen_US
dc.titleThe Development and Characterization of Three-Colour Fluorescence Cross-Correlation Spectroscopy
dc.typemaster thesis
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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