Engineering Improved Seed Degreening in Brassica napus (canola)

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atmire.migration.oldid5229
dc.contributor.advisorSamuel, Marcus
dc.contributor.authorPerkins, Mendel
dc.contributor.committeememberHarker, Neil
dc.contributor.committeememberChua, Gordon
dc.contributor.committeememberMuench, Douglas
dc.date.accessioned2017-01-06T18:58:36Z
dc.date.available2017-01-06T18:58:36Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractFrost during Brassica napus (canola) embryo development increases seed chlorophyll content at maturity causing losses to canola producers. In Arabidopsis, it was demonstrated that the transcription factor, ABI3, regulates genes responsible for seed maturation and de-greening. During seed maturation, ABI3 is required for the activation of the SGR2 (stay-green) which drives degreening and overexpression of ABI3 was sufficient to impart frost-tolerant degreening. Given the high sequence similarity between Arabidopsis and canola, this system can be easily translated into canola. ABI3 homolog isolated from canola was able to complement Arabidopsis abi3-6 mutant, indicating conserved function. Canola transgenics overexpressing ABI3 were developed and were shown to promote significant degreening of seeds despite frost exposure. These transgenic lines also did not exhibit any deleterious pleiotropic effects. This study provides evidence for how enhanced expression of ABI3 confers frost-tolerant degreening, a trait that could be easily incorporated into canola hybrids.en_US
dc.identifier.citationPerkins, M. (2017). Engineering Improved Seed Degreening in Brassica napus (canola) (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25895en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25895
dc.identifier.urihttp://hdl.handle.net/11023/3550
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyScience
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBotany
dc.subjectBiology--Molecular
dc.subjectPlant Physiology
dc.subject.otherbiotechnology
dc.subject.othercanola
dc.subject.otherdegreening
dc.subject.otherstress tolerance
dc.titleEngineering Improved Seed Degreening in Brassica napus (canola)
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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