Fluid Flow Induced Modulation of Smad2 and Akt Phosphorylation in Arterial Endothelial Cells
| atmire.migration.oldid | 635 | |
| dc.contributor.advisor | Rinker, Kristina | |
| dc.contributor.author | Obrejanu, Alexander | |
| dc.date.accessioned | 2013-01-25T18:11:56Z | |
| dc.date.available | 2013-06-15T07:01:39Z | |
| dc.date.issued | 2013-01-25 | |
| dc.date.submitted | 2013 | en |
| dc.description.abstract | Endothelial cells, which line the artery walls, respond to fluid flow stimulation through activation of signalling pathways. Smad2, a signalling molecule and transcription factor typically associated with TGF-β signalling, is preferentially phosphorylated in the linker region (L- pSmad2) and localized to the nucleus in a flow-dependent manner in endothelial cells. Here, we show that L-pSmad2 expression is higher in the high shear stress region of the thoracic aorta than in the lower shear stress region of the lesser curvature of mice. L-pSmad2 was not affected by flow pulsatility in human endothelial cells and did not vary in disturbed flow versus laminar regions in carotid arteries of surgically modified mice. Smad2 siRNA treatment of human endothelial cells resulted in decreased Smad2 and increased protein levels of Akt under flow. These findings represent novel contributions to the knowledge of fluid flow-induced Smad2 signalling and its role in endothelial health. | en_US |
| dc.identifier.citation | Obrejanu, A. (2013). Fluid Flow Induced Modulation of Smad2 and Akt Phosphorylation in Arterial Endothelial Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26196 | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/26196 | |
| dc.identifier.uri | http://hdl.handle.net/11023/484 | |
| dc.language.iso | eng | |
| dc.publisher.faculty | Graduate Studies | |
| dc.publisher.institution | University of Calgary | en |
| dc.publisher.place | Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | |
| dc.subject | Engineering--Biomedical | |
| dc.subject.classification | Fluid-flow | en_US |
| dc.subject.classification | Smad2 | en_US |
| dc.subject.classification | Endothelial | en_US |
| dc.title | Fluid Flow Induced Modulation of Smad2 and Akt Phosphorylation in Arterial Endothelial Cells | |
| dc.type | master thesis | |
| thesis.degree.discipline | Biomedical Engineering | |
| thesis.degree.grantor | University of Calgary | |
| thesis.degree.name | Master of Science (MSc) | |
| ucalgary.item.requestcopy | true |