The retinal pigment epithelium (RPE) is a vital component of the vertebrate eye and is a pigment rich epithelial cell layer that performs multiple functions. The RPE shares a common neural origin with the retina but turns of different genes during specification. While embryonic RPE development is characterized, there is limited knowledge of RPE development post-optic cup morphogenesis, and how potential heterogeneity emerges within RPE cells. In this project, I use the zebrafish model to uncover the presence of diversity within the developing RPE, and the developmental trajectories of potential RPE subpopulations. I also explore the proliferative capacity of the identified RPE subpopulations, as it is known that RPE grows throughout the life of the zebrafish, however the process has not been described. This research shows the distribution and spatial localization of potential proliferative subpopulations of the RPE with the help of a transcription factor, tfec. Specifically, I used scRNA sequencing data to identify candidate genes to classify RPE cell subpopulations, then validated their expression via in-situ techniques in wild type zebrafish retinas. To identify potential proliferative differences within these subpopulations I employed EdU and immunohistochemical analysis on transgenic zebrafish (Tg(tfec:egfp)). My data suggest, for the first time, that the zebrafish RPE layer is heterogeneous. The three annotated RPE clusters appeared to align with distinct developmental stages of the RPE cells. The clusters were localized to specific anatomical regions, with mature RPE cells concentrated in the central retina, differentiating RPE cells in the intermediate zone and most naïve cells in the peripheral RPE. Finally, data from this study suggests that the central RPE zone is most highly proliferative, where RPE progenitors are scattered in amongst mature RPE cells. Overall, my research provides initial insights into the spatial and temporal expression of three RPE subpopulations within the zebrafish retina. Data also implicates a new, proliferative role of the central RPE. These findings could be the foundation for future work exploring gene-specific roles within the clusters using loss/gain-of function experiments. Ultimately, this research contributes to the knowledge gaps in zebrafish RPE development and progenitor biology and leads to new avenues for research.