Infection of the Gut by HIV-1: The Pathogenic Role of the Nef Protein

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atmire.migration.oldid459
dc.contributor.advisorvan Marle, Guido
dc.contributor.authorGrant, Tannika
dc.date.accessioned2012-12-03T18:47:36Z
dc.date.available2013-06-15T07:01:36Z
dc.date.issued2012-12-03
dc.date.submitted2012en
dc.description.abstractHuman Immunodeficiency virus type 1 (HIV-1) targets several cells in the immune system and induces their progressive decline and the Acquired Immunodeficiency Syndrome (AIDS). HIV produces viral proteins including Nef; an important multifunctional protein and viral pathogenic determinant. The gastrointestinal tract and impairment of its epithelial barrier has been implicated in HIV pathogenesis, thus an investigation of the contribution of HIV-1 Nef on intestinal barrier dysfunction was performed. An in vitro model system was established and then used to analyse the pathogenic role of Nef. Trans-epithelial electrical resistance, Real time (RT)-PCR and fluorescence microscopy methods were used. It was found that Nef expression in colonic Caco-2 cells, Jurkat and U937 cells; reduced expression of the Caspases, increased viability of cells, while decreasing expression of the tight junction proteins. Thus HIV-1 Nef protein may contribute to HIV pathogenesis by disrupting barrier integrity and cell death of colonic epithelial cells and monolayers.en_US
dc.identifier.citationGrant, T. (2012). Infection of the Gut by HIV-1: The Pathogenic Role of the Nef Protein (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26512en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26512
dc.identifier.urihttp://hdl.handle.net/11023/331
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subjectVirology
dc.subject.classificationHIV-1en_US
dc.subject.classificationNefen_US
dc.subject.classificationGuten_US
dc.subject.classificationIn Vitroen_US
dc.subject.classificationCaco-2en_US
dc.subject.classificationCo-cultureen_US
dc.subject.classificationEpithelial cellsen_US
dc.subject.classificationTrans-epithelial electrical resistanceen_US
dc.subject.classificationReal-time PCRen_US
dc.subject.classificationCloningen_US
dc.subject.classificationFluorescence microscopyen_US
dc.subject.classificationJurkaten_US
dc.subject.classificationU937en_US
dc.subject.classificationBarrier integrityen_US
dc.subject.classificationCell deathen_US
dc.subject.classificationCaspasesen_US
dc.subject.classificationTight junctionsen_US
dc.titleInfection of the Gut by HIV-1: The Pathogenic Role of the Nef Protein
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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