Tumour-Suppressor Knockdown as a Strategy for Peripheral Nerve Regeneration: The Effects of PTEN and Rb

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atmire.migration.oldid4676
dc.contributor.advisorZochodne, Douglas
dc.contributor.authorEaton, Shane
dc.contributor.committeememberOusman, Shalina
dc.contributor.committeememberWhelan, Patrick
dc.contributor.committeememberSchuurmans, Carol
dc.date.accessioned2016-07-26T18:24:36Z
dc.date.available2016-07-26T18:24:36Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractTumour-suppressors, such as PTEN and Rb, function to limit growth and proliferation. In post-mitotic peripheral sensory neurons, their knockdown may be utilized to promote regenerative growth. The goal of this thesis was to evaluate the effects of PTEN and Rb knockdown on peripheral nerve regeneration. Towards this goal I first addressed technical challenges, improving on a neuronal culture and immunostaining protocol. In assessing Rb knockdown I found increases in neuritic branching, but use of the PTEN inhibitor bpV(pic) was ineffective on neurite outgrowth, and therefore my results with combined Rb knockdown and PTEN inhibition were inconclusive. I also evaluated the phenotype of a PTEN conditional knockout mouse where PTEN is deleted from peripheral sensory neurons and found that they exhibited enlarged nerves with supernumerary myelinated axon profiles and a corresponding increase in SNAP amplitudes. Loss of phenotype was observed in a subsequent generation, pointing to complications in the Cre-lox conditional knockout system.en_US
dc.identifier.citationEaton, S. (2016). Tumour-Suppressor Knockdown as a Strategy for Peripheral Nerve Regeneration: The Effects of PTEN and Rb (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28532en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28532
dc.identifier.urihttp://hdl.handle.net/11023/3155
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectBiology--Molecular
dc.subjectNeuroscience
dc.subject.classificationPTENen_US
dc.subject.classificationRben_US
dc.subject.classificationRetinoblastomaen_US
dc.subject.classificationTumour-suppressoren_US
dc.subject.classificationRegenerationen_US
dc.subject.classificationAxonen_US
dc.subject.classificationNerveen_US
dc.subject.classificationPNSen_US
dc.subject.classificationNeuronen_US
dc.subject.classificationDRGen_US
dc.subject.classificationCell Cultureen_US
dc.subject.classificationDorsal Root Ganglionen_US
dc.subject.classificationSciaticen_US
dc.titleTumour-Suppressor Knockdown as a Strategy for Peripheral Nerve Regeneration: The Effects of PTEN and Rb
dc.typemaster thesis
thesis.degree.disciplineNeuroscience
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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