Comparative and functional analysis of β-adrenoceptors in human myometrium

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dc.contributor.advisorGiembycz, Mark A
dc.contributor.advisorSlater, Donna M.
dc.contributor.authorAlbadri, Randa G. A.
dc.contributor.committeememberCole, William C.
dc.contributor.committeememberKlein, Claudia
dc.contributor.committeemembervon der Weid, Pierre-Yves
dc.date2018-06
dc.date.accessioned2018-01-31T22:40:42Z
dc.date.available2018-01-31T22:40:42Z
dc.date.issued2018-01
dc.description.abstractThere are three subtypes of the β-adrenoceptors (β1, β2 and β3). All the β-adrenoceptor subtypes couple to Gαs subunit of G protein. All the β-adrenoceptor subtypes couple mainly to Gαs subunit of G protein. β2-adrenoceptor agonists have been used clinically to suppress myometrial contractions during the management of preterm labour. β2-adrenoceptor agonists lose their effectiveness in a short time and their use causes several side effects. Evidence in the literature supports the expression of functional β2- and β3-adrenoceptors in the lower pregnant human myometrial tissues. However, no definitive understanding in terms of regional and temporal expression and function of the β-adrenoceptors in the uterus was obtained by reviewing the literature. Experiments of this work have confirmed the expression of the three β-adrenoceptor subtypes in term pregnant (upper and lower) human myometrial tissues by real-time RT-PCR analysis. Poor antibody specificity was an obstacle to determine and localize the β-adrenoceptor proteins in the myometrium by immunohistochemistry. β2- and β3-adrenoceptor agonists did not affect the expression of CRISPLD2, RGS2 and NA4A3 in primary MSM cells after 2 and 6 h of incubation. Further research must be done to determine and localize the expression of the β-adrenoceptors in the myometrium.en_US
dc.identifier.citationAlbadri, R. G. A. (2018). Comparative and functional analysis of β-adrenoceptors in human myometrium (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/5447en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/5447
dc.identifier.urihttp://hdl.handle.net/1880/106366
dc.language.isoeng
dc.publisher.facultyCumming School of Medicine
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectβ-adrenoceptors
dc.subjectmyometrium
dc.subject.classificationPhysiologyen_US
dc.subject.classificationPharmacologyen_US
dc.titleComparative and functional analysis of β-adrenoceptors in human myometrium
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
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