Selective Separation of Polar Unsaturated Organics Using a Water Stationary Phase in Gas Chromatography

Marno Jones, Amberley

Selective Separation of Polar Unsaturated Organics Using a Water Stationary Phase in Gas Chromatography

dc.contributor.advisor	Thurbide, Kevin
dc.contributor.author	Marno Jones, Amberley
dc.contributor.committeemember	Gailer, Jurgen
dc.contributor.committeemember	Achari, Gopal
dc.contributor.committeemember	Thangadurai, Venkataraman
dc.date	2022-06
dc.date.accessioned	2022-05-06T18:10:13Z
dc.date.available	2022-05-06T18:10:13Z
dc.date.issued	2022-04
dc.description.abstract	In this thesis, a water stationary phase is explored as a novel means of selectively separating unsaturated analytes in gas chromatography. Several unsaturated/saturated analyte pairs consisting mainly of alcohols and carboxylic acids were examined using both a 30 m conventional non-polar HP-5 column and an 11 m water phase column. For most investigated on the HP-5 column, analytes often eluted very close to each other (~6-12 seconds apart) with poor resolution. By comparison, the shorter water phase column well separated each of the analogue pairs by about 3-8 minutes or more. As well on the water phase, analytes with a triple bond were much more retained than those with two double bonds, which in turn were much more retained than those with one double bond. Conversely, on the HP-5 column these were poorly separated if at all. Next, the addition of various metal ion salts to the water phase was explored. While Ca2+ ion produced modest increases in selectivity, the addition of Ag+ ion was most influential and further increased the original water phase selectivity by a factor of 2.3. This also illustrated the flexibility of the system to be adjusted for such separations as a variety of metal ion salts could be easily dissolved into the water stationary phase for this investigation. Lastly, an exploration of unsaturated analyte separations found that cis/trans isomers were baseline resolved on the water phase but co-eluted on a 30 m conventional polar Carbowax column. Similarly, positional isomers varying the location of the double bond were found to separate with a selectivity value near 1.1 on the Carbowax column, whereas on the water phase column they yielded a value of 1.3 and eluted in the reverse order. The mechanistic implications of OH---pi bonding in the water phase was discussed as a potential origin for the selectivity observed. The method was applied to gasoline, model contaminated water, essential oil, and food stuff analysis. Results indicate that this method could be a very useful means of selectively separating such unsaturated analytes.	en_US
dc.identifier.citation	Marno Jones, A. (2022). Selective separation of polar unsaturated organics using a water stationary phase in gas chromatography (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/39746
dc.identifier.uri	http://hdl.handle.net/1880/114627
dc.language.iso	eng	en_US
dc.publisher.faculty	Science	en_US
dc.publisher.institution	University of Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.	en_US
dc.subject.classification	Chemistry--Analytical	en_US
dc.title	Selective Separation of Polar Unsaturated Organics Using a Water Stationary Phase in Gas Chromatography	en_US
dc.type	master thesis	en_US
thesis.degree.discipline	Chemistry	en_US
thesis.degree.grantor	University of Calgary	en_US
thesis.degree.name	Master of Science (MSc)	en_US
ucalgary.item.requestcopy	true	en_US