Protecting the gut against Clostridium difficile: A role for Keratinocyte growth factor

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atmire.migration.oldid2301
dc.contributor.advisorBeck, Paul
dc.contributor.advisorMacDonald, Justin
dc.contributor.authorAlhassan, Basmah Faris
dc.date.accessioned2014-07-18T14:47:08Z
dc.date.embargolift2016-07-17T14:47:08Z
dc.date.issued2014-07-18
dc.date.submitted2014en
dc.description.abstractClostridium difficle (Cdiff) infection (CDI) causes severe colitis via its toxins: toxin A and toxin B (TcdAB), inducing barrier disruption, inflammation and cell death. Current treatments are failing and the need to search for new targets is urgent. Several host factors have shown to modulate CDI in animals and patients. Intestinal growth factors are a major part of the mucosal host response in the gut. Among them, keratinocyte growth factor (KGF) has been shown to be protective in many colitis models. In this thesis, the protective role of KGF was demonstrated against Cdiff toxin injury. In vitro, KGF protected Caco-2 cells from barrier disruption and cell death induced by TcdAB. Exogenous KGF administration protected mice from acute intestinal toxin damage. Interestingly, KGF deletion did not impact the acute toxin-induced colitis in mice; however, endogenous KGF was essential for normal recovery from TcdAB-induced colitis as KGF−/− mice demonstrated impaired recovery after 24-48 hours post TcdAB exposure. Findings from this study may lead to identifying a cause for the variability in clinical response among patients with CDI as well as new therapeutic targets for this devastating disease.en_US
dc.description.embargoterms2 yearsen_US
dc.identifier.citationAlhassan, B. F. (2014). Protecting the gut against Clostridium difficile: A role for Keratinocyte growth factor (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27468en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27468
dc.identifier.urihttp://hdl.handle.net/11023/1644
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectImmunology
dc.subjectMedicine and Surgery
dc.subjectPathology
dc.subject.classificationClostridium difficileen_US
dc.subject.classificationKeratinocyte growth factoren_US
dc.subject.classificationKGFen_US
dc.subject.classificationColitisen_US
dc.subject.classificationIntestinal epithelial cellsen_US
dc.subject.classificationClostridium difficile toxinsen_US
dc.subject.classificationIntestinal trefoil factoren_US
dc.titleProtecting the gut against Clostridium difficile: A role for Keratinocyte growth factor
dc.typemaster thesis
thesis.degree.disciplineGastrointestinal Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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