Investigating the role of the cell envelope in E. coli relating to silver sensitivity and resistance

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dc.contributor.advisorTurner, Raymond Joseph
dc.contributor.authorWestersund, Connor
dc.contributor.committeememberGedamu, Lashitew
dc.contributor.committeememberHarrison, Joe J.
dc.date2018-11
dc.date.accessioned2018-05-17T21:09:39Z
dc.date.available2018-05-17T21:09:39Z
dc.date.issued2018-05-11
dc.description.abstractSilver (I) is an antimicrobial agent that has established antimicrobial activity, yet the mechanism of action is unclear. This thesis follows up on an observation that Ag+ ions cause the cell membrane to separate from the cell wall in Escherichia coli. Data from a chemical genomic screen was utilized, identifying 6 Ag-responsive cells with single gene deletions (damX, rodZ, minC, sanA, ybhO, tolB), which are genes involved in maintenance of the cell envelope. When these mutants were grown in the presence of Ag+, cells demonstrated extensive cell envelope damage as seen by transmission electron microscopy. Evaluation of kill curves, some mutants conferred resistance and others sensitivity to silver in comparison to wildtype. From the data collected, it was recognized that functions around cell wall and transport across the cell membranes have roles in both silver resistance and sensitivity.en_US
dc.identifier.citationWestersund, C. (2018). Investigating the role of the cell envelope in E. coli relating to silver sensitivity and resistance (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/31922en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/31922
dc.identifier.urihttp://hdl.handle.net/1880/106641
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyScience
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectSilver
dc.subjectSilver ions
dc.subjectAntimicrobials
dc.subject.classificationEducation--Healthen_US
dc.subject.classificationEducation--Sciencesen_US
dc.subject.classificationBiologyen_US
dc.subject.classificationMicrobiologyen_US
dc.titleInvestigating the role of the cell envelope in E. coli relating to silver sensitivity and resistance
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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