Subtyping Virulence Genes of Pathogenic Escherichia coli Isolated from Beef Cattle and Clinical Cases in Alberta

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atmire.migration.oldid4204
dc.contributor.advisorDeVinney, Rebekah
dc.contributor.advisorStanford, Kimberly
dc.contributor.advisorReuter, Tim
dc.contributor.authorFerreira Tostes, Renata
dc.contributor.committeememberDeVinney, Rebekah
dc.contributor.committeememberStanford, Kimberly
dc.contributor.committeememberReuter, Tim
dc.contributor.committeememberKastelic, John
dc.contributor.committeememberGannon, Victor
dc.date.accessioned2016-05-05T15:28:54Z
dc.date.available2016-05-05T15:28:54Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractThe objective of this study was to subtype virulence genes of pathogenic E. coli isolated from cattle and human clinical cases in western Canada. We identified a variety of virulence profiles among strains and most serogroups presented a dominant genotype. Many O157 and non-O157 isolates lacked stx genes; the absence was greater in non-O157 and O157:nonH7 than in O157:H7 strains. Overall, virulence profile of cattle isolates were very similar to those reported in clinical cases, although it was not possible to establish a clear relationship between strains’ genotype and season collected or place-of-origin. Monitoring stx (e.g. stx1a and stx2a) and eae (e.g. λ/γ1-eae) subtypes can be a valuable tool for risk assessment and prediction of disease outcome. Microbial contamination may occur throughout the food production chain. Therefore, monitoring pathogenic E. coli is crucial to ensure food safety across the farm-to-fork scope.en_US
dc.identifier.citationFerreira Tostes, R. (2016). Subtyping Virulence Genes of Pathogenic Escherichia coli Isolated from Beef Cattle and Clinical Cases in Alberta (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27931en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27931
dc.identifier.urihttp://hdl.handle.net/11023/2972
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subjectPublic Health
dc.subject.classificationSubtypingen_US
dc.subject.classificationShiga-toxinen_US
dc.subject.classificationIntiminen_US
dc.subject.classificationSTECen_US
dc.subject.classificationPyrosequencingen_US
dc.subject.classificationGenotypeen_US
dc.subject.classificationEscherichia colien_US
dc.titleSubtyping Virulence Genes of Pathogenic Escherichia coli Isolated from Beef Cattle and Clinical Cases in Alberta
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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