Novel copper-containing membrane monooxygenases (CuMMOs) encoded by alkane-utilizing Betaproteobacteria

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dc.contributor.authorRochman, Fauziah F.
dc.contributor.authorKwon, Miye
dc.contributor.authorKhadka, Roshan
dc.contributor.authorTamas, Ivica
dc.contributor.authorLopez-Jauregui, Abraham
dc.contributor.authorSheremet, Andriy
dc.contributor.authorSmirnova, Angela V.
dc.contributor.authorMalmstrom, Rex
dc.contributor.authorYoon, Sukhwan
dc.contributor.authorWoyke, Tanja
dc.contributor.authorDunfield, Peter F.
dc.contributor.authorVerbeke, Tobin J.
dc.date.accessioned2019-12-04T18:12:58Z
dc.date.available2019-12-04T18:12:58Z
dc.date.issued2019-07-01
dc.description.abstractCopper-containing membrane monooxygenases (CuMMOs) are encoded by xmoCAB(D) gene clusters and catalyze the oxidation of methane, ammonia, or some short chain alkanes and alkenes. In a metagenome constructed from an oilsands tailings pond we detected an xmoCABD gene cluster with <59% derived amino acid identity to genes from known bacteria. Stable isotope probing experiments combined with a specific xmoA qPCR assay demonstrated that the bacteria possessing these genes were incapable of methane assimilation, but did grow on ethane and propane. Single-cell genomes (SAGs) from propane-enriched samples were therefore constructed and screened with the specific PCR assay to identify bacteria possessing the target gene cluster. Multiple SAGs of Betaproteobacteria belonging to the genera Rhodoferax and Polaromonas possessed close homologues of the metagenomic xmoCABD gene cluster. Unexpectedly, each of these two genera also possessed other xmoCABD paralogs, representing two additional lineages in phylogenetic analyses. Metabolic reconstructions from SAGs predicted that neither bacterium was capable of catabolic methane or ammonia oxidation, but that both were capable of higher n-alkane degradation. The involvement of the encoded CuMMOs in alkane oxidation was further suggested by reverse transcription PCR analyses, which detected elevated transcription of the xmoA genes upon enrichment of water samples with propane as the sole energy source. Enrichments, isotope incorporation studies, genome reconstructions, and gene expression studies therefore all agreed that the unknown xmoCABD operons did not encode methane or ammonia monooxygenases, but rather n-alkane monooxygenases. This study broadens the known diversity of CuMMOs and identifies non-nitrifying Betaproteobacteria as possessing these enzymes.en_US
dc.description.grantingagencyNatural Sciences and Engineering Research Council (NSERC)en_US
dc.identifier.citationRochman, F. F., Kwon, M., Khadka, R., Tamas, I., Lopez-Jauregui, A. A., Sheremet, A., … Verbeke, T. J. (2019). Novel copper-containing membrane monooxygenases (CuMMOs) encoded by alkane-utilizing Betaproteobacteria. The ISME Journal. doi: 10.1038/s41396-019-0561-2en_US
dc.identifier.doihttps://doi.org/10.1038/s41396-019-0561-2en_US
dc.identifier.grantnumber2014-05067en_US
dc.identifier.issn1751-7362
dc.identifier.urihttp://hdl.handle.net/1880/111309
dc.identifier.urihttps://doi.org/10.11575/PRISM/46156
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.publisher.departmentBiological Sciencesen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://www.nature.com/ismej/authors-and-refereesen_US
dc.rightsUnless otherwise indicated, this material is protected by copyright and has been made available with authorization from the copyright owner. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectmethaneen_US
dc.subjectalkaneen_US
dc.subjectpropaneen_US
dc.subjectCUMMOen_US
dc.titleNovel copper-containing membrane monooxygenases (CuMMOs) encoded by alkane-utilizing Betaproteobacteriaen_US
dc.typejournal articleen_US
dc.typesubmittedVersionen_US
ucalgary.item.requestcopytrueen_US
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