Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio)

Shah, Soham Manoj

Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio)

dc.contributor.advisor	Habibi, Hamid R.
dc.contributor.advisor	Achari, Gopal
dc.contributor.author	Shah, Soham Manoj
dc.contributor.committeemember	Gieg, Lisa Marie
dc.contributor.committeemember	Ruckstuhl, K. E.
dc.date	2018-11
dc.date.accessioned	2018-07-31T15:14:08Z
dc.date.available	2018-07-31T15:14:08Z
dc.date.issued	2018-07-30
dc.description.abstract	Sulfolane is a widely used polar, aprotic solvent for multiple applications in numerous industries. Through problems with storage, leaks, spills and leeching; sulfolane has been introduced into the environment and has been detected through chemical analysis in groundwater and creeks around the world including Alberta, Canada (800 µg·mL-1), Louisiana, USA (2900 µg·mL-1) and Brisbane, Australia (4344 µg·mL-1). Previous research on terrestrial organisms has demonstrated hyperactivity, shortness of breath, convulsions, and internal molecular changes following sulfolane exposure. However, relatively little information is available on the adverse impacts of sulfolane on aquatic organisms. This study investigates the adverse effects of sulfolane on morphological, behavioural and transcript abundance in developing zebrafish embryos. A wide range of concentrations (0 – 5000 µg·mL-1) were tested on survival, hatching, morphometric characteristics (yolk sac and pericardial edema, hemorrhaging, spinal malformations, swim bladder inflation), growth (larval length, condition factor, eye volume), yolk sac utilization, behavior (response to touch stimuli and light stimuli) and transcript abundances of a number of genes (ahr1a, cyp1a, thraa, dio1, dio2, dio3, 11βhsd2, gr, aqp3a, cyp19a1b, ddc, gria2b, hsp70). Minimum effective doses were different for morphometrics and growth parameters (800-1000 µg·mL-1), behaviour (200-500 µg·mL-1) and transcript abundance (10 µg·mL-1). The overall results provide novel information on the adverse effects of sulfolane on an aquatic vertebrate model organism, and a framework for better understanding of potential adverse health impacts of environmental levels of sulfolane in fish and other vertebrates.	en_US
dc.identifier.citation	Shah, S. M. (2018). Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio) (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32716	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/32716
dc.identifier.uri	http://hdl.handle.net/1880/107535
dc.language.iso	eng
dc.publisher.faculty	Graduate Studies
dc.publisher.faculty	Science
dc.publisher.institution	University of Calgary	en
dc.publisher.place	Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject	Sulfolane
dc.subject	Zebrafish
dc.subject	Danio rerio
dc.subject	Morphometrics
dc.subject	Transcript Abundance
dc.subject	Behavioural Assessment
dc.subject	Larval Activity
dc.subject	Remediation
dc.subject	Development
dc.subject	Health Impact Assessment
dc.subject.classification	Animal Physiology	en_US
dc.subject.classification	Zoology	en_US
dc.subject.classification	Toxicology	en_US
dc.title	Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio)
dc.type	master thesis
thesis.degree.discipline	Biological Sciences
thesis.degree.grantor	University of Calgary
thesis.degree.name	Master of Science (MSc)
ucalgary.item.requestcopy	true