Browsing by Author "Jorgenson, Kristen"

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    Development of suspension culture protocols to expand populations of adult human stem cells from synovial fluid
    (2012) Jorgenson, Kristen; Sen, Arindom
    Synovial fluid (SF) contains a population of mesenchymal stem cells (MSCs) with the capacity to generate cartilage, and as such may play a role is new treatment approaches for cartilage disorders. However, it is necessary to generate clinically-relevant numbers of these cells in a scalable and reproducible culture system. This study used three donors to evaluate microcarrier technology to expand populations of adult human SF­derived MSCs. Inoculation and growth protocols developed showed that an increase in cell to microcarrier ratio and using a fed batch culture system had the greatest impact in increasing the final cell yield. With the improved suspension culture protocol, a maximum cell density of 1.89 x 105 cells/ml was achieved compared with 1.12 x 105 cells/ml obtained in static culture. Additionally, serial propagation in microcarrier suspension bioreactors achieved a 21.3 cell fold increase after 18 days in culture. Characterization of SF-derived MSCs showed that the cells maintained their defining characteristics.