Development of suspension culture protocols to expand populations of adult human stem cells from synovial fluid
Date
2012
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Abstract
Synovial fluid (SF) contains a population of mesenchymal stem cells (MSCs) with the capacity to generate cartilage, and as such may play a role is new treatment approaches for cartilage disorders. However, it is necessary to generate clinically-relevant numbers of these cells in a scalable and reproducible culture system. This study used three donors to evaluate microcarrier technology to expand populations of adult human SFÂderived MSCs. Inoculation and growth protocols developed showed that an increase in cell to microcarrier ratio and using a fed batch culture system had the greatest impact in increasing the final cell yield. With the improved suspension culture protocol, a maximum cell density of 1.89 x 105 cells/ml was achieved compared with 1.12 x 105 cells/ml obtained in static culture. Additionally, serial propagation in microcarrier suspension bioreactors achieved a 21.3 cell fold increase after 18 days in culture. Characterization of SF-derived MSCs showed that the cells maintained their defining characteristics.
Description
Bibliography: p. 150-156
Many pages are in colour.
Many pages are in colour.
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Citation
Jorgenson, K. (2012). Development of suspension culture protocols to expand populations of adult human stem cells from synovial fluid (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4832