Development of suspension culture protocols to expand populations of adult human stem cells from synovial fluid

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dc.contributor.advisorSen, Arindom
dc.contributor.authorJorgenson, Kristen
dc.date.accessioned2017-12-18T22:32:56Z
dc.date.available2017-12-18T22:32:56Z
dc.date.issued2012
dc.descriptionBibliography: p. 150-156en
dc.descriptionMany pages are in colour.en
dc.description.abstractSynovial fluid (SF) contains a population of mesenchymal stem cells (MSCs) with the capacity to generate cartilage, and as such may play a role is new treatment approaches for cartilage disorders. However, it is necessary to generate clinically-relevant numbers of these cells in a scalable and reproducible culture system. This study used three donors to evaluate microcarrier technology to expand populations of adult human SF­derived MSCs. Inoculation and growth protocols developed showed that an increase in cell to microcarrier ratio and using a fed batch culture system had the greatest impact in increasing the final cell yield. With the improved suspension culture protocol, a maximum cell density of 1.89 x 105 cells/ml was achieved compared with 1.12 x 105 cells/ml obtained in static culture. Additionally, serial propagation in microcarrier suspension bioreactors achieved a 21.3 cell fold increase after 18 days in culture. Characterization of SF-derived MSCs showed that the cells maintained their defining characteristics.
dc.format.extentxxvii, 163 leaves : ill. ; 30 cm.en
dc.identifier.citationJorgenson, K. (2012). Development of suspension culture protocols to expand populations of adult human stem cells from synovial fluid (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4832en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4832
dc.identifier.urihttp://hdl.handle.net/1880/105833
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleDevelopment of suspension culture protocols to expand populations of adult human stem cells from synovial fluid
dc.typemaster thesis
thesis.degree.disciplineChemical and Petroleum Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2088 627942960
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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