Browsing by Author "Moo, Eng Kuan"

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    5th Rocky Mountain Muscle Symposium
    (Faculty of Kinesiology, 2023-05-19) Joumaa, Venus; Hessel, Anthony; Nishikawa, Kiisa; Millard, Matthew; Han, Seong-won; Bell, Kaylyn; Leonard, Tim; Kaya, Motoshi; Corr, David; Swank, Douglas; Corvelyn, Marlies; Sahani, Ridhi; Mazara, Nicole; Moo, Eng Kuan; Granzier, Henk; Oldshue, Ashley; Adkins, Amy; Loya, Amy; Boldt, Kevin; Desloovere, Kaat; Fukutani, Atsuki; Martino, Giovanni; De Beukelaer, Nathalie; Horslen, Brian; Agen, Anouk; Costamanga, Domiziana; Swank, Doug; Holt, Natalie
    Proceedings from the 4th Rocky Mountain Muscle Symposium, Canmore Nordic Centre, June 19-21, 2023.
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    In vivo Sarcomere Lengths and Sarcomere Elongations Are Not Uniform across an Intact Muscle
    (Frontiers in Physiology, 2016-05) Herzog, Walter; Moo, Eng Kuan; Fortuna, Rafael; Sibole, Scott C.; Abusara, Ziad
    Sarcomere lengths have been a crucial outcome measure for understanding and explaining basic muscle properties and muscle function. Sarcomere lengths for a given muscle are typically measured at a single spot, often in the mid-belly of the muscle, and at a given muscle length. It is then assumed implicitly that the sarcomere length measured at this single spot represents the sarcomere lengths at other locations within the muscle, and force-length, force-velocity, and power-velocity properties of muscles are often implied based on these single sarcomere length measurements. Although, intuitively appealing, this assumption is yet to be supported by systematic evidence. The objective of this study was to measure sarcomere lengths at defined locations along and across an intact muscle, at different muscle lengths. Using second harmonic generation (SHG) imaging technique, sarcomere patterns in passive mouse tibialis anterior (TA) were imaged in a non-contact manner at five selected locations (“proximal,” “distal,” “middle,” “medial,” and “lateral” TA sites) and at three different lengths encompassing the anatomical range of motion of the TA. We showed that sarcomere lengths varied substantially within small regions of the muscle and also for different sites across the entire TA. Also, sarcomere elongations with muscle lengthening were non-uniform across the muscle, with the highest sarcomere stretches occurring near the myotendinous junction. We conclude that muscle mechanics derived from sarcomere length measured from a small region of a muscle may not well-represent the sarcomere length and associated functional properties of the entire muscle.
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    Proceedings of the 4th Rocky Mountain Muscle Symposium
    (2019-07-27) Joumaa, Venus; Hessel, Anthony; Nishikawa, Kiisa; Millard, Matthew; Han, Seong-won; Bell, Kaylyn; Leonard, Tim; Corr, David; Swank, Douglas; Corvelyn, Marlies; Sahani, Ridhi; Mazara, Nicole; Moo, Eng Kuan; Lieber, Rick; Kaya, Motoshi; Granzier, Henk; Oldshue, Ashley; Adkins, Amy; Loya, Amy; Boldt, Kevin; Desloovere, Kaat; Fukutani, Atsuki; Martino, Giovanni; De Beukelaer, Nathalie; Horslen, Brian; Agten, Anouk; Costamagna, Domiziana; Lee, Sabrina; Schwaner, Marie Janneke; Abbott, Emily; Binder-Markey, Benjamin; Siebert, Tobias; Lin, David; Fontana, Heiliane de Brito; Daley, Monica; Hahn, Daniel; Evangelidis, Pavlos; Knaus, Katherine; Smith, Ian; Deloovere, Kat; Kawakami, Yasuo; Hodson-Tole, Emma; Oliveira, Liliam; Ichinose, Hoshizora; Tillin, Neale; Raiteri, Brent; Seiberl, Wolfgang; Vaz, Marco; Cruz-Montecinos, Carlos; Griffiths, Robert; MacIntosh, Brian; Lourdes Rios, Jacqueline; Bossuyt, Fransiska
    Proceedings from the 4th Rocky Mountain Muscle Symposium, Canmore Nordic Centre, July 27-29, 2019
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    Unfolding of membrane ruffles of in situ chondrocytes under compressive loads
    (Journal of Orthopaedic Research, 2016) Herzog, Walter; Moo, Eng Kuan
    Impact loading results in chondrocyte death. Previous studies implicated high tensile strain rates in chondrocyte membranes as the cause of impact-induced cell deaths. However, this hypothesis relies on the untested assumption that chondrocyte membranes unfold in vivo during physiological tissue compression, but do not unfold during impact loading. Although membrane unfolding has been observed in isolated chondrocytes during osmotically induced swelling and mechanical compression, it is not known if membrane unfolding also occurs in chondrocytes embedded in their natural extracellular matrix. This study was aimed at quantifying changes in membrane morphology of in situ superficial zone chondrocytes during slow physiological cartilage compression.