Gene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease”
Date
2014-02-05
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Abstract
Current diagnostic tools lack sensitivity to detect JD early after infection. Hence, this study aimed to find biomarkers for early diagnosis of JD. An infection trial was set up with a high dose (n=5) and low dose (n=5) oral challenge with Mycobacterium avium subsp. paratuberculosis at 2 weeks of age and 5 non-infected control animals. Whole blood was collected at a 3-month interval (3, 6, 9, 12 and 15 months) for gene expression analysis using Affymetrix® GeneChip® Bovine Genome Array on samples obtained at 3, 6 and 9 months after infection. Microarray findings were confirmed using qPCR and longitudinal assays profiling transcripts over 15-month period was carried out. This study found differential gene expression at each sampled time point and found transcripts for potential biomarker use. CD46 was upregulated and BOLA and BNBD9-like genes were downregulated over the entire 15-months. Overall immune response was inhibited in MAP-challenged animals.
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Microbiology, Biology--Molecular
Citation
David, J. (2014). Gene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease” (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26265