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Interaction of Silica Nanoparticles with Primary Alveolar Epithelial Cells

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Advisor
Amrein, Matthias
Author
Bogari, Nawaf N.
Accessioned
2015-09-01T16:19:20Z
Issued
2015-09-01
Submitted
2015
Other
Nanoparticles
Alveolar epithelial cells
Primary cell culture
Endocytosis
Subject
Education--Health
Biology--Cell
Physiology
Health Sciences
Immunology
Type
Thesis
Metadata
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Abstract
The fate of nanoparticles reaching the alveolar lung is not completely understood. Clearance of these particles has been ascribed to the alveolar macrophages scavenging this region. In contrast, in my thesis, I show in vitro, that particles interact more strongly with the alveolar epithelial cells (AECs) than alveolar macrophages (AMs), are then taken up efficiently and transported across the cells and released on the basolateral side. First, I developed a protocol for obtaining a pure culture of rat AEC. Single cell force spectroscopy showed the AEC to respond strongly and in a clathrin-independent manner to the particles, unlike a host of control cells, including alveolar macrophages. Fluorescence light microscopy and total internal reflection fluorescence light microscopy (TIRF) demonstrated the transport to be novel, actin-dependent and microtubule independent. In summary, my thesis provides evidence of a second clearance mechanism in addition to AMs.
Corporate
University of Calgary
Faculty
Graduate Studies
Doi
http://dx.doi.org/10.5072/PRISM/26596
Uri
http://hdl.handle.net/11023/2417
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