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dc.contributor.authorCavey, Michael J.en
dc.contributor.authorWong, Gene K.en
dc.date.accessioned2006-08-14T20:02:06Z
dc.date.available2006-08-14T20:02:06Z
dc.date.issued1992
dc.identifier.citationGENE K. WONG AND MICHAEL J. CAVEY "Development of the Liver in the Chicken Embryo. I. HEPATIC CORDS AND SlNUSOlDS" THE ANATOMICAL RECORD 234555-567 (1992)en
dc.identifier.issn1552-4884
dc.identifier.urihttp://hdl.handle.net/1880/43417
dc.descriptionCopyright held by publisheren
dc.description.abstractHemopoiesis in the liver of the chicken embryo begins on day 7 of incubation (Hamburger and Hamilton Stage 30) and peaks on day 14 (Stage 40). During this time frame, the differentiation of hepatic cells was examined by light microscopy, transmission and scanning electron microscopy, and morphometry. The avian liver is a closely packed mass of dendriform cords and discontinuous sinusoids. Hepatocytes are pyramidal in shape, and they ring the bile canaliculi which run through the centers of the cords. Semithin sections, made possible by infiltration and embedding in glycol methacrylate, were stained with hematoxylin and eosin to assess the general architecture of the organ and the lipid content of the hepatocytes and by the periodic acid-Schiff reaction and hematoxylin to visualize the cytoplasmic stores of glycogen. The number of hepatocytes with demonstrable glycogen fluctuates erratically in early hemopoiesis, and the proportion of glycogen-containing cells progressively increases as hemopoiesis climbs to a peak. Most differentiating hepatocytes are devoid of lipid droplets until Stages 39 and 40. From Stage 30 to 35, hepatocyte volume falls to its lowest value. Subsequently (Stages 36 to 40), cell volume increases and hepatocytes achieve a relatively uniform size. Ultrastructural changes in the differentiating hepatocytes, including alterations to the mitochondria, endoplasmic reticulum, and Golgi apparatus, are documented. These morphological and morphometric findings on the prehepatocyte population and hepatic vasculature cover 2 of the 3 elements deemed critical to hepatic hemopoiesis in many vertebrates. o 1992 Wiley-Liss, Inc.en
dc.format.extent2079727 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.subjectBiologyen
dc.subject.otherCapillariesen
dc.subject.otherChick embryoen
dc.subject.otherVascular endotheliumen
dc.subject.otherHematopoiesisen
dc.subject.otherKupffer cellsen
dc.subject.otherLiveren
dc.subject.otherMicroscopyen
dc.subject.otherMorphogenesisen
dc.titleDevelopment of the liver in the chicken embryo. I. Hepatic cords and sinusoidsen
dc.typejournal article
dc.description.refereedyesen
dc.contributor.institutionUniversity of Calgaryen
dc.contributor.facultyFaculty of Scienceen
dc.contributor.departmentBiological Sciencesen
dc.publisher.urlhttp://www3.interscience.wiley.com/cgi-bin/jhome/28243
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/35099


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