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Model of minor stroke with mild peri-infarct ischemic injury

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Author
Tuor, Ursula I.
Deng, Qinbo
Rushforth, Dave
Foniok, Taduesz
Qiao, Min
Accessioned
2016-05-27T18:20:15Z
Available
2016-05-27T18:20:15Z
Issued
2016-04-29
Subject
Photothrombosis
Peri-infarct
Mild ischemic injury
Magnetic resonance imaging
Type
journal article
Metadata
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Abstract
Background Transient ischemic attack, minor stroke and stroke recurrence need improved treatment but lack animal models for research. The aim was to modify photothrombosis methods thereby producing both a minor stroke (with adjacent mild damage) or a minor recurrent stroke. New method A minor stroke, as detected using magnetic resonance imaging and histology, was produced using a low intensity beam of white light with a bright centre, a low dose of Rose Bengal and a short 5 min illumination of thinned skull. A recurrent minor stroke was produced by repeating the procedure two days later except the cortical mask was positioned 1.5 mm posteriorly. Results The minor photothrombosis procedure produced a small superficial infarct surrounded by a region of scattered necrosis detected histologically. Marked hyperintensities in diffusion weighted and T2 images identified the infarct. Peri-infarct regions with modest T2 increases corresponded to regions of scattered cell death. A recurrent minor photothrombosis produced additional damage in regions with overlapping mild injury. Comparison with existing methods Previous photothrombosis methods usually produce large cortical infarcts with little penumbra. The current method produces small infarcts with diffuse mild peri-infarct ischemic injury that can be diagnosed using T2 imaging. Conclusions The modified photothrombotic procedure will produce a minor stroke consisting of a small infarct in a region with marked diffusion and T2 hyperintensities and a peri-infarct region of selective necrosis with modest T2 changes. Minor recurrent stroke is readily produced but imaging is key for assessing size and location of each insult. Abbreviations Dw, diffusion weighted; MRI, magnetic resonance imaging; Iba1, Ionized Calcium-Binding Adapter Molecule 1; T2w, transverse relaxation time weighted; TIA, transient ischemic attack
Grantingagency
Canadian Institutes for Health Research, Brain Canada
Refereed
Yes
Publisher's version of article deposited according to Elsevier Open access policy https://www.elsevier.com/about/company-information/policies/open-access-licenses May 27, 2016
 
Citation
Tuor UI, Deng Q, Rushforth D, Foniok T, Qiao M. (2016). Model of minor stroke with mild peri-infarct ischemic injury. Journal of Neuroscience Methods, 268, 56-65. doi: 10.1016/j.jneumeth.2016.04.025
Corporate
University of Calgary
Department
Department of Clinical Neurosciences, Department of Physiology and Pharmacology
Faculty
Medicine
Institution
University of Calgary
Url
https://www.elsevier.com/
Publisher
Elsevier
Doi
http://dx.doi.org/10.11575/PRISM/33750
Uri
http://hdl.handle.net/1880/51247
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