Construction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResT

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dc.contributor.authorBandy, Nicken_US
dc.date.accessioned2013-08-29T20:51:14Z
dc.date.embargolift10000-01-01
dc.date.issued2013-08-29
dc.description.abstractDuring DNA replication in Borrelia burgdorferi, replication of linear elements results in an inverted dimer repeat of the telomere which is subsequently resolved by the telomere resolvase, ResT. ResT has been demonstrated as essential and a knock-out strain has never been produced. Using a conditional expression system a resT knock-out strain has been produced and used to investigate the effects of ResT depletion of spirochete viability as well as genome stability. ResT depleted spirochetes ceased to divide after ResT depletion, however, remained viable up to 16 days suggesting an arrested state of the spirochetes. Southern blots of the DNA revealed a shift of linear plasmid DNA to a higher molecular weight representing the circular intermediate. DNA replication did not continue in spirochetes after ResT depletion suggesting ResT may play a larger role in replication than previously thought. en_US
dc.description.embargotermsforeveren_US
dc.description.refereedYesen_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/10760
dc.identifier.urihttp://hdl.handle.net/1880/49807
dc.language.isoengen_US
dc.publisher.corporateUniversity of Calgaryen_US
dc.publisher.facultyFaculty of Medicineen_US
dc.titleConstruction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResTen_US
dc.typeThesisen_US
thesis.degree.disciplineDepartment of Biochemistry and Molecular Biologyen_US
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