TNF-α mediated upregulation of NaV1.7 currents in rat dorsal root ganglion neurons is independent of CRMP2 SUMOylation

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dc.contributor.authorde Macedo, Flávio H P
dc.contributor.authorAires, Rosária D
dc.contributor.authorFonseca, Esdras G
dc.contributor.authorFerreira, Renata C M
dc.contributor.authorMachado, Daniel P D
dc.contributor.authorChen, Lina
dc.contributor.authorZhang, Fang-Xiong
dc.contributor.authorSouza, Ivana A
dc.contributor.authorLemos, Virgínia S
dc.contributor.authorRomero, Thiago R L
dc.contributor.authorMoutal, Aubin
dc.contributor.authorKhanna, Rajesh
dc.contributor.authorZamponi, Gerald W
dc.contributor.authorCruz, Jader S
dc.date.accessioned2020-01-05T01:02:25Z
dc.date.available2020-01-05T01:02:25Z
dc.date.issued2019-12-30
dc.date.updated2020-01-05T01:02:25Z
dc.description.abstractAbstract Clinical and preclinical studies have shown that patients with Diabetic Neuropathy Pain (DNP) present with increased tumor necrosis factor alpha (TNF-α) serum concentration, whereas studies with diabetic animals have shown that TNF-α induces an increase in NaV1.7 sodium channel expression. This is expected to result in sensitization of nociceptor neuron terminals, and therefore the development of DNP. For further study of this mechanism, dissociated dorsal root ganglion (DRG) neurons were exposed to TNF-α for 6 h, at a concentration equivalent to that measured in STZ-induced diabetic rats that developed hyperalgesia. Tetrodotoxin sensitive (TTXs), resistant (TTXr) and total sodium current was studied in these DRG neurons. Total sodium current was also studied in DRG neurons expressing the collapsin response mediator protein 2 (CRMP2) SUMO-incompetent mutant protein (CRMP2-K374A), which causes a significant reduction in NaV1.7 membrane cell expression levels. Our results show that TNF-α exposure increased the density of the total, TTXs and TTXr sodium current in DRG neurons. Furthermore, TNF-α shifted the steady state activation and inactivation curves of the total and TTXs sodium current. DRG neurons expressing the CRMP2-K374A mutant also exhibited total sodium current increases after exposure to TNF-α, indicating that these effects were independent of SUMOylation of CRMP2. In conclusion, TNF-α sensitizes DRG neurons via augmentation of whole cell sodium current. This may underlie the pronociceptive effects of TNF-α and suggests a molecular mechanism responsible for pain hypersensitivity in diabetic neuropathy patients.
dc.identifier.citationMolecular Brain. 2019 Dec 30;12(1):117
dc.identifier.doihttps://doi.org/10.1186/s13041-019-0538-0
dc.identifier.urihttp://hdl.handle.net/1880/111430
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.titleTNF-α mediated upregulation of NaV1.7 currents in rat dorsal root ganglion neurons is independent of CRMP2 SUMOylation
dc.typeJournal Article
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