MicroRNA miR-223 modulates NLRP3 and Keap1, mitigating lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells and murine mammary glands

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dc.contributor.authorZhou, Man
dc.contributor.authorBarkema, Herman W.
dc.contributor.authorGao, Jian
dc.contributor.authorYang, Jingyue
dc.contributor.authorWang, Yue
dc.contributor.authorKastelic, John P.
dc.contributor.authorKhan, Sohrab
dc.contributor.authorLiu, Gang
dc.contributor.authorHan, Bo
dc.date.accessioned2023-09-17T00:03:48Z
dc.date.available2023-09-17T00:03:48Z
dc.date.issued2023-09-14
dc.date.updated2023-09-17T00:03:48Z
dc.description.abstractAbstract Bovine mastitis, the most prevalent and costly disease in dairy cows worldwide, decreases milk quality and quantity, and increases cow culling. However, involvement of microRNAs (miRNAs) in mastitis is not well characterized. The objective was to determine the role of microRNA-223 (miR-223) in regulation of the nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome and kelch like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) oxidative stress pathway in mastitis models induced by lipopolysaccharide (LPS) treatment of immortalized bovine mammary epithelial cells (bMECs) and murine mammary glands. In bMECs cultured in vitro, LPS-induced inflammation downregulated bta-miR-223; the latter interacted directly with the 3’ untranslated region (3’ UTR) of NLRP3 and Keap1. Overexpression of bta-miR-223 in bMECs decreased LPS and Adenosine 5’-triphosphate (ATP)-induced NLRP3 and its mediation of caspase 1 and IL-1β, and inhibited LPS-induced Keap1 and Nrf2 mediated oxidative stress, whereas inhibition of bta-miR-223 had opposite effects. In an in vivo murine model of LPS-induced mastitis, increased miR-223 mitigated pathology in the murine mammary gland, whereas decreased miR-223 increased inflammatory changes and oxidative stress. In conclusion, bta-miR-223 mitigated inflammation and oxidative injury by downregulating the NLRP3 inflammasome and Keap1/Nrf2 signaling pathway. This study implicated bta-miR-223 in regulation of inflammatory responses, with potential as a novel target for treating bovine mastitis and other diseases.
dc.identifier.citationVeterinary Research. 2023 Sep 14;54(1):78
dc.identifier.urihttps://doi.org/10.1186/s13567-023-01206-5
dc.identifier.urihttps://hdl.handle.net/1880/117083
dc.identifier.urihttps://doi.org/10.11575/PRISM/41927
dc.language.rfc3066en
dc.rights.holderL’Institut National de Recherche en Agriculture, Alimentation et Environnement (INRAE)
dc.titleMicroRNA miR-223 modulates NLRP3 and Keap1, mitigating lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells and murine mammary glands
dc.typeJournal Article
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