Detecting Deoxyhemoglobin in Spinal Cord Vasculature of the Experimental Autoimmune Encephalomyelitis Mouse Model of Multiple Sclerosis Using Susceptibility MRI and Hyperoxygenation

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dc.contributor.authorNathoo, Nabeela
dc.contributor.authorRogers, James A.
dc.contributor.authorYong, V. Wee
dc.contributor.authorDunn, Jeff F.
dc.date.accessioned2015-08-05T18:57:44Z
dc.date.available2015-08-05T18:57:44Z
dc.date.issued2015-05-18
dc.description.abstractSusceptibility-weighted imaging (SWI) detects hypointensities due to iron deposition and deoxyhemoglobin. Previously it was shown that SWI detects hypointensities in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS), most of which are due to intravascular deoxyhemoglobin, with a small proportion being due to iron deposition in the central nervous system parenchyma and demyelination. However, animals had to be sacrificed to differentiate these two types of lesions which is impractical for time course studies or for human application. Here, we proposed altering the inspired oxygen concentration during imaging to identify deoxyhemoglobin-based hypointensities in vivo. SWI was performed on lumbar spinal cords of naive control and EAE mice using 30% O2 then 100% O2. Some mice were imaged using 30% O2, 100% O2 and after perfusion. Most SWI-visible hypointensities seen with 30% O2 changed in appearance upon administration of 100% O2, and were not visible after perfusion. That hypointensities changed with hyperoxygenation indicates that they were caused by deoxyhemoglobin. We show that increasing the inspired oxygen concentration identifies deoxyhemoglobin-based hypointensities in vivo. This could be applied in future studies to investigate the contribution of vascular-based hypointensities with SWI in EAE and MS over time.en_US
dc.description.refereedYesen_US
dc.identifier.citationNathoo N, Rogers JA, Yong VW, Dunn JF (2015) Detecting Deoxyhemoglobin in Spinal Cord Vasculature of the Experimental Autoimmune Encephalomyelitis Mouse Model of Multiple Sclerosis Using Susceptibility MRI and Hyperoxygenation. PLoS ONE 10(5): e0127033. doi:10.1371/journal.pone.0127033en_US
dc.identifier.doi10.1371/journal.pone.0127033
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/33451
dc.identifier.urihttp://hdl.handle.net/1880/50831
dc.language.isoenen_US
dc.publisherPLOS ONEen_US
dc.publisher.corporateUniversity of Calgary
dc.publisher.departmentRadiologyen_US
dc.publisher.facultyMedicineen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127033en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMagnetic resonance imagingen_US
dc.subjectOxygenen_US
dc.subjectMultiple sclerosisen_US
dc.subjectSpinal corden_US
dc.subjectMiceen_US
dc.subjectIn vivo imagingen_US
dc.subjectAnimal behavioren_US
dc.subjectCentral nervous systemen_US
dc.titleDetecting Deoxyhemoglobin in Spinal Cord Vasculature of the Experimental Autoimmune Encephalomyelitis Mouse Model of Multiple Sclerosis Using Susceptibility MRI and Hyperoxygenationen_US
dc.typejournal article
thesis.degree.disciplineClinical Neurosciences
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