Pericytes as mediators of infiltration of macrophages in multiple sclerosis
| dc.contributor.author | Kaushik, Deepak K. | |
| dc.contributor.author | Bhattacharya, Anindita | |
| dc.contributor.author | Lozinski, Brian M. | |
| dc.contributor.author | Wee Yong, V. | |
| dc.date.accessioned | 2021-12-26T01:02:18Z | |
| dc.date.available | 2021-12-26T01:02:18Z | |
| dc.date.issued | 2021-12-24 | |
| dc.date.updated | 2021-12-26T01:02:18Z | |
| dc.description.abstract | Abstract Background Multiple sclerosis (MS) is a neurodegenerative condition of the central nervous system (CNS). It is associated with blood–brain barrier (BBB) breakdown and intravasation of leukocytes, particularly monocyte-derived macrophages, into the CNS. Pericytes are mural cells that are encased within the basement membrane of vasculature, and they contribute functionally to the neurovascular unit. These cells play an important role in maintaining BBB integrity and CNS homeostasis. However, the critical role of pericytes in mediating inflammation in MS or its models is unclear. Whether pericytes infiltrate into the CNS parenchyma in MS also needs clarification. Methods CNS samples from the experimental autoimmune encephalomyelitis (EAE) mouse model of MS were collected at different time points for immunohistochemical analysis of pericytes along the inflamed vasculature. These findings were validated using MS brain specimens, and further analysis of pericyte involvement in inflammation was carried out by culturing primary pericytes and macrophages. Multiplex ELISA, transmigration assay and real-time PCR were used to study the inflammatory potential of pericytes in cultures. Results We found that pericytes exhibit a heterogenous morphology, with notable elongation in the inflamed perivascular cuffs of EAE. This was manifested by a decrease in pericyte density but an increase in the coverage by pericytes along the vasculature. Chondroitin sulfate proteoglycans (CSPGs), a family of extracellular matrix proteins enriched within inflamed perivascular cuffs, elevated levels of pro-inflammatory chemokines/cytokines in pericytes in culture. Importantly, pericytes stimulated with CSPGs enhanced macrophage migration. We did not detect pericytes in the CNS parenchyma during EAE, and this was corroborated in MS brain samples. Conclusions Our data suggest that pericytes seek to restore the BBB through increased coverage, but that their exposure to CSPGs prompt their facilitation of macrophages to enter the CNS to elevate neuroinflammation in EAE and MS. | |
| dc.identifier.citation | Journal of Neuroinflammation. 2021 Dec 24;18(1):301 | |
| dc.identifier.doi | https://doi.org/10.1186/s12974-021-02358-x | |
| dc.identifier.uri | http://hdl.handle.net/1880/114240 | |
| dc.identifier.uri | https://doi.org/10.11575/PRISM/44306 | |
| dc.language.rfc3066 | en | |
| dc.rights.holder | The Author(s) | |
| dc.title | Pericytes as mediators of infiltration of macrophages in multiple sclerosis | |
| dc.type | Journal Article |